现货Sigma货号P0899-500mg多聚-D-赖氨酸氢溴suān盐(规格包装500mg/瓶)(目录价¥11522.33元/瓶)13611631389上海睿安生物
聚-D-赖氨酸 氢_溴_酸盐
suitable for cell culture,Mol wt 70000-150000
别名:D-Lysine homopolymer hydrobromide
应用:
多聚-D-赖氨酸氢溴suān盐被用于以下研究:
●动物细胞培养
●大鼠皮层神经元培养
●细胞培养和siRNA处理
●原代细胞培养和成人神经元网络模型
●皮质星形胶质细胞培养
●免疫荧光染色[将HDF细胞接种到多聚-D-赖氨酸(P0899,Sigma-Aldrich)包被的玻璃盖玻片上]
●NPC收集和培养条件
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5-1.0ml的该产品0.1mg/mL溶液用作细胞培养的基质对25cm²进行涂覆。较低分子量的该产品粘度相对较低,但较高分子量的形式则可提供每分子更多的附着位点。
包装:10 mg in glass bottle;50mg,100mg,500mg in poly bottle;1g in poly bottle。
生化/生理作用:该产品是一种细胞的非特异性附着因子,可用于通过增强细胞膜的带负电离子与培养表面之间的静电相互作用而促进细胞与固体基质的粘附。当被细胞培养表面吸收后,聚-D-赖氨酸可增加带正电的细胞结合位点数量。
组分:聚-D-赖氨酸是一种带正电的氨基酸聚合物,其中每个赖氨酸残基约有一个HBr。氢溴suān盐可使聚-D-赖氨酸呈可溶于水的结晶形式。在β结构中可能会发现少量的产物,因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意:无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
分析说明:
本品的分子量为70000-150000。为了除去HBr,将本品溶解在中性缓冲液中并透析除去盐。通常,本品用作粘附因子,将50ml无菌组织培养级水加至5mg多聚赖氨酸中,无菌条件下,每25cm²表面包被1ml。包被5分钟,然后吸去溶液并充分清表面。晾干2小时,然后加入细胞和培养基。
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