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- YB636Mu01
- 2025年07月16日
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- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)
- 库存：
10000
- 供应商：
钰博生物
酸性核磷蛋白32A(ANP32A)重组蛋白
适用生物 Mus musculus (Mouse，小鼠)
酸性核磷蛋白32A(ANP32A)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 55.2kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
ybJ376Hu01 几丁质酶3样蛋白2(CHI3L2)重组蛋白 Recombinant Chitinase 3 Like Protein 2 (CHI3L2) Homo sapiens (Human)
ybJ399Hu01 糖磺基转移酶9(CHST9)重组蛋白 Recombinant Carbohydrate Sulfotransferase 9 (CHST9) Homo sapiens (Human)
ybJ410Hu01 钙调宁蛋白2(CNN2)重组蛋白 Recombinant Calponin 2 (CNN2) Homo sapiens (Human)
ybJ419Hu01 钙调宁蛋白1(CNN1)重组蛋白 Recombinant Calponin 1, Basic (CNN1) Homo sapiens (Human)
ybJ447Mu01 染色质装配因子1亚基B(CHAF1B)重组蛋白 Recombinant Chromatin Assembly Factor 1, Subunit B (CHAF1B) Mus musculus (Mouse)
ybJ484Mu01 胱硫醚β合酶(CβS)重组蛋白 Recombinant Cystathionine Beta Synthase (CbS) Mus musculus (Mouse)
ybJ495Hu01 戴帽蛋白肌Z系β(CAPZβ)重组蛋白 Recombinant Capping Protein Muscle Z Line Beta (CAPZb) Homo sapiens (Human)
ybJ512Hu01 内酰胺酶β(LACTβ)重组蛋白 Recombinant Lactamase Beta (LACTb) Homo sapiens (Human)
ybJ516Hu01 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白 Recombinant Lecithin Cholesterol Acyltransferase (LCAT) Homo sapiens (Human)
ybJ541Hu01 Necdin蛋白(NDN)重组蛋白 Recombinant Necdin (NDN) Homo sapiens (Human)
ybJ545Ra01 Bcl2/腺病毒E1B相互作用蛋白3(BNIP3)重组蛋白 Recombinant Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) Rattus norvegicus (Rat)
ybJ548Hu01 BMP结合内皮调节因子(BMPER)重组蛋白 Recombinant BMP Binding Endothelial Regulator (BMPER) Homo sapiens (Human)
ybJ554Hu01 桥连整合因子2(BIN2)重组蛋白 Recombinant Bridging Integrator 2 (BIN2) Homo sapiens (Human)
ybJ555Hu01 桥连整合因子1(BIN1)重组蛋白 Recombinant Bridging Integrator 1 (BIN1) Homo sapiens (Human)
ybJ557Hu01 苄氯素1(BECN1)重组蛋白 Recombinant Beclin 1 (BECN1) Homo sapiens (Human)
适用生物 Mus musculus (Mouse，小鼠)
酸性核磷蛋白32A(ANP32A)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 55.2kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | RPJ636Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 55.2kDa |
| Predicted isoelectric point | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Glu2-Asp247 (Accession # O35381) with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- EMDKRIYLE LRNRTPSDVK ELVLDNCKSI EGKIEGLTDE FEELEFLSTI NVGLTSISNL PKLNKLKKLE LSENRISGDL EVLAEKCPNL KHLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLNA YRENVFKLLP QVMYLDGYDR DNKEAPDSDV EGYVEDDDEE DEDEEEYDEY AQLVEDEEEE DEEEEGEEED VSGEEEEDEE GYNDGEVDDE EDEEEAGEEE GSQKRKREPD DEGEEDD
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- EMDKRIYLE LRNRTPSDVK ELVLDNCKSI EGKIEGLTDE FEELEFLSTI NVGLTSISNL PKLNKLKKLE LSENRISGDL EVLAEKCPNL KHLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLNA YRENVFKLLP QVMYLDGYDR DNKEAPDSDV EGYVEDDDEE DEDEEEYDEY AQLVEDEEEE DEEEEGEEED VSGEEEEDEE GYNDGEVDDE EDEEEAGEEE GSQKRKREPD DEGEEDD
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
酸性核磷蛋白32A(ANP32A)重组蛋白 Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
酸性核磷蛋白32A(ANP32A)重组蛋白 Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybJ374Mu01 几丁质酶1(CHIT1)重组蛋白 Recombinant Chitinase 1 (CHIT1) Mus musculus (Mouse)
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
ybJ376Hu01 几丁质酶3样蛋白2(CHI3L2)重组蛋白 Recombinant Chitinase 3 Like Protein 2 (CHI3L2) Homo sapiens (Human)
ybJ399Hu01 糖磺基转移酶9(CHST9)重组蛋白 Recombinant Carbohydrate Sulfotransferase 9 (CHST9) Homo sapiens (Human)
ybJ410Hu01 钙调宁蛋白2(CNN2)重组蛋白 Recombinant Calponin 2 (CNN2) Homo sapiens (Human)
ybJ419Hu01 钙调宁蛋白1(CNN1)重组蛋白 Recombinant Calponin 1, Basic (CNN1) Homo sapiens (Human)
ybJ447Mu01 染色质装配因子1亚基B(CHAF1B)重组蛋白 Recombinant Chromatin Assembly Factor 1, Subunit B (CHAF1B) Mus musculus (Mouse)
ybJ484Mu01 胱硫醚β合酶(CβS)重组蛋白 Recombinant Cystathionine Beta Synthase (CbS) Mus musculus (Mouse)
ybJ495Hu01 戴帽蛋白肌Z系β(CAPZβ)重组蛋白 Recombinant Capping Protein Muscle Z Line Beta (CAPZb) Homo sapiens (Human)
ybJ512Hu01 内酰胺酶β(LACTβ)重组蛋白 Recombinant Lactamase Beta (LACTb) Homo sapiens (Human)
ybJ516Hu01 卵磷脂胆固醇脂酰转移酶(LCAT)重组蛋白 Recombinant Lecithin Cholesterol Acyltransferase (LCAT) Homo sapiens (Human)
ybJ541Hu01 Necdin蛋白(NDN)重组蛋白 Recombinant Necdin (NDN) Homo sapiens (Human)
ybJ545Ra01 Bcl2/腺病毒E1B相互作用蛋白3(BNIP3)重组蛋白 Recombinant Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) Rattus norvegicus (Rat)
ybJ548Hu01 BMP结合内皮调节因子(BMPER)重组蛋白 Recombinant BMP Binding Endothelial Regulator (BMPER) Homo sapiens (Human)
ybJ554Hu01 桥连整合因子2(BIN2)重组蛋白 Recombinant Bridging Integrator 2 (BIN2) Homo sapiens (Human)
ybJ555Hu01 桥连整合因子1(BIN1)重组蛋白 Recombinant Bridging Integrator 1 (BIN1) Homo sapiens (Human)
ybJ557Hu01 苄氯素1(BECN1)重组蛋白 Recombinant Beclin 1 (BECN1) Homo sapiens (Human)
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文献和实验相关实验
的表达系统会造成质粒的不稳定，细胞生长速度的下降和重组蛋白产量的降低[32，33]。Lanzer和Bujard曾对常用的lac启动子-操纵子系统进行了广泛的研究，证明操纵子放在启动子序列的不同位置会造成70倍差异的抑制。将17bp的操纵子置于-10和-35六聚体区之间所形成的抑制比将其放在-35区的上游或-10区的下游要高50-70倍[34]。 启动子的第三个特性是其简便和廉价的可诱导性。大量生产蛋白质最常用的启动子是热诱导（λPL）和化学诱导(trp)启动子。异丙基硫代-β-D-半乳糖
基进行共价修饰，但用尿素溶解具有不电离，呈中性，成本低，蛋白质复性后除去不会造成大量蛋白质沉淀以及溶解的包涵体可选用多种色谱法纯化等优点，故目前已被广泛采用。 盐酸胍溶解能力达95%以上，且溶解作用快而不造成重组蛋白质的共价修饰。但它也有成本高、在酸性条件下易产生沉淀、复性后除去可能造成大量蛋白质沉淀和对蛋白质离子交换色谱有干扰等缺点。 包涵体最后的纯化，一般是离子交换，疏水，或者是亲和层析，亲和层析尤其是金属螯合层析用得比较广泛，而纯化的效果也不错，通常是一步就能达到纯度为95%以上的包涵
交换柱，来把可溶的sigma 分离了出来。为什么这一次用Poros HS 50 而不是前面用的阴离 子交换柱呢？因为sarkosyl带负电，会与阴离子交换柱结合得很好。再加上sigma32 很奇怪，既有负电的表面，又有带正电的表面，所以可以用阳离子交换柱。值得一提的是绝大部分蛋白质都是偏酸性，所以阴离子交换柱用的比阳离子交换柱频繁得多。 好了，聊完离子交换柱，现在回到Dr Burgess要我们作的另一个很重要的实验。 Sigma32在大肠杆菌过表达的时候，绝大部分都是inclusion
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酸性核磷蛋白32A(ANP32A)重组蛋白
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