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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
翌圣生物科技(上海)股份有限公司
- 库存:
大量
- 英文名:
Myc-tag,Mouse mAb
- 保质期:
有效期1年
- 保存条件:
-20℃保存
- 规格:
20μl(30601ES20)/100 μL(30601ES60)
| 规格: | 20μl(30601ES20) | 产品价格: | ¥248.00 |
|---|---|---|---|
| 规格: | 100 μL(30601ES60) | 产品价格: | ¥748.00 |
| 产品名称 | 产品编号 | 规格 |
| Myc-tag , Mouse mAb | 30601ES20 | 20 μL |
| 30601ES60 | 100 μL |
产品简介
c-Myc 蛋白是一种位于细胞核内的转录因子,参与细胞增殖、分化和凋亡等生理过程。研究表明,在胃癌、乳腺癌、
结肠癌和宫颈癌等许多肿瘤组织或细胞中,c-Myc 蛋白的表达量要明显高于正常组织和细胞中的表达量。Myc 标签(EQKLISEEDL)是来自 c-Myc 蛋白的一段多肽,由于分子量很小,不会影响融合蛋白的生物特性,广泛用于检测细菌、酵母菌、昆虫和哺乳动物细胞等系统中融合蛋白的表达。Myc 标签可以放在 C 端或 N 端,Myc 融合蛋白可通过偶联 Myc 标签抗体到二乙-烯砜活化的琼脂糖上而进行亲和纯化,但 c-Myc 融合蛋白的低 pH 洗脱条件往往会降低蛋白质的活力,因此c-Myc 标签系统多应用于检测但很少用于纯化。本抗体可用于检测带 Myc 标签的融合蛋白及天然 c-Myc 蛋白。
产品应用
建议稀释浓度:WB:1:1000-1:10000; IP: 1/500;
产品信息
| 产品类型(Product Type) | Mouse mAb |
| 纯化方式(Purification method) | Affinity Purified |
| 免疫原(Immunogen) | Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (EQKLISEEDL) coupled to KLH. |
| 应用(Application) | WB,IP |
| 储存缓冲液(Storage Buffer) | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
储存条件
-25~-15℃保存
注意事项
1. 避免反复冻融。
2. 本产品仅作科研用途。
3. 为了您的安全和健康,请穿实验服并佩戴一次性手套操作。
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文献和实验[2] Cheng Y, Zhu W, Ding C, et al. IRF7 Is Involved in Both STING and MAVS Mediating IFN-β Signaling in IRF3-Lacking Chickens. J Immunol. 2019;203(7):1930-1942. doi:10.4049/jimmunol.1900293(IF:4.718)
[3] Zhuang W, Yan Z. The S2-S3 Loop of Kv7.4 Channels Is Essential for Calmodulin Regulation of Channel Activation. Front Physiol. 2021;11:604134. Published 2021 Jan 20. doi:10.3389/fphys.2020.604134(IF:4.566)
[4] Wang X, Guo J, Che X, Jia R. PCBP1 inhibits the expression of oncogenic STAT3 isoform by targeting alternative splicing of STAT3 exon 23. Int J Biol Sci. 2019;15(6):1177-1186. Published 2019 May 7. doi:10.7150/ijbs.33103(IF:4.067)
[5] Che X, Liu M, Li D, Li Z, Guo J, Jia R. RAN and YBX1 are required for cell proliferation and IL-4 expression and linked to poor prognosis in oral squamous cell carcinoma. Exp Cell Res. 2021;406(2):112767. doi:10.1016/j.yexcr.2021.112767(IF:3.905)
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse









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