- ¥80
- 上海钰博
- 进口/国产
- YB830Hu01
- 2025年11月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Recombinant Transglutaminase 2, Tissue (TGM2)
- 库存：
10000
- 供应商：
钰博生物
组织转谷氨酰胺酶(TGM2)重组蛋白
适用生物 Homo sapiens (Human，人)
组织转谷氨酰胺酶(TGM2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 78.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
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yb-4234R ABATγ氨基丁酸转氨酶抗体 浓度1mg/ml
yb-6513R A2BP1共济失调蛋白2结合蛋白1抗体 浓度1mg/ml
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yb-6510R ABH8AlkB同源蛋白8抗体 浓度1mg/ml
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yb-11383R Acetoacetyl-CoA synthetase脂肪酰辅酶A家族成员1抗体 浓度1mg/ml
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yb-11448R phospho-AANAT (Thr29)磷酸化芳香胺N-乙酰化转移酶抗体 浓度1mg/ml
yb-11449R ABLIM1肌动蛋白结合蛋白1抗体 浓度1mg/ml
yb-11450R AMIGO2粘附分子IgG样结构域蛋白2抗体 浓度1mg/ml
yb-11451R Advillin肌动蛋白结合蛋白DOC6抗体 浓度1mg/ml
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yb-11705R AIP1激活素受体相互作用蛋白1抗体 浓度1mg/ml
yb-11706R AKR7A2醛固酮类还原酶家族7成员A2抗体 浓度1mg/ml
yb-6951R AKT1+2+3蛋白激酶AKT1,2,3抗体 浓度1mg/ml
适用生物 Homo sapiens (Human，人)
组织转谷氨酰胺酶(TGM2)重组蛋白
产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 78.8kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Transglutaminase 2, Tissue (TGM2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | RPB830Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 78.8kDa |
| Predicted isoelectric point | 5.3 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Subcellular Location | n/a |
| Residues | Met1~Ala687 (Accession # P21980) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.1% sarcosyl and preservative. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW LTLHFEGRNY EASVDSLTFS VVTGPAPSQE AGTKARFPLR DAVEEGDWTA TVVDQQDCTL SLQLTTPANA PIGLYRLSLE ASTGYQGSSF VLGHFILLFN AWCPADAVYL DSEEERQEYV LTQQGFIYQG SAKFIKNIPWNFGQFEDGIL DICLILLDVN PKFLKNAGRD CSRRSSPVYV GRVVSGMVNC NDDQGVLLGR WDNNYGDGVS PMSWIGSVDI LRRWKNHGCQ RVKYGQCWVF AAVACTVLRC LGIPTRVVTN YNSAHDQNSN LLIEYFRNEF GEIQGDKSEM IWNFHCWVES WMTRPDLQPG YEGWQALDPT PQEKSEGTYC CGPVPVRAIK EGDLSTKYDA PFVFAEVNAD VVDWIQQDDG SVHKSINRSL IVGLKISTKS VGRDEREDIT HTYKYPEGSS EEREAFTRAN HLNKLAEKEE TGMAMRIRVG QSMNMGSDFD VFAHITNNTA EEYVCRLLLC ARTVSYNGIL GPECGTKYLL NLNLEPFSEK SVPLCILYEK YRDCLTESNL IKVRALLVEP VINSYLLAER DLYLENPEIK IRILGEPKQK RKLVAEVSLQ NPLPVALEGC TFTVEGAGLT EEQKTVEIPD PVEAGEEVKV RMDLLPLHMG LHKLVVNFES DKLKAVKGFR NVIIGPA
MGHHHHHHSGSEF-MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW LTLHFEGRNY EASVDSLTFS VVTGPAPSQE AGTKARFPLR DAVEEGDWTA TVVDQQDCTL SLQLTTPANA PIGLYRLSLE ASTGYQGSSF VLGHFILLFN AWCPADAVYL DSEEERQEYV LTQQGFIYQG SAKFIKNIPWNFGQFEDGIL DICLILLDVN PKFLKNAGRD CSRRSSPVYV GRVVSGMVNC NDDQGVLLGR WDNNYGDGVS PMSWIGSVDI LRRWKNHGCQ RVKYGQCWVF AAVACTVLRC LGIPTRVVTN YNSAHDQNSN LLIEYFRNEF GEIQGDKSEM IWNFHCWVES WMTRPDLQPG YEGWQALDPT PQEKSEGTYC CGPVPVRAIK EGDLSTKYDA PFVFAEVNAD VVDWIQQDDG SVHKSINRSL IVGLKISTKS VGRDEREDIT HTYKYPEGSS EEREAFTRAN HLNKLAEKEE TGMAMRIRVG QSMNMGSDFD VFAHITNNTA EEYVCRLLLC ARTVSYNGIL GPECGTKYLL NLNLEPFSEK SVPLCILYEK YRDCLTESNL IKVRALLVEP VINSYLLAER DLYLENPEIK IRILGEPKQK RKLVAEVSLQ NPLPVALEGC TFTVEGAGLT EEQKTVEIPD PVEAGEEVKV RMDLLPLHMG LHKLVVNFES DKLKAVKGFR NVIIGPA
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
组织转谷氨酰胺酶(TGM2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
组织转谷氨酰胺酶(TGM2)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
yb-9790R AFAP微丝相关蛋白1抗体 浓度1mg/ml
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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yb-4236R ADAM17肿瘤坏死因子α转换酶 浓度1mg/ml
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yb-5709R ALDH1L1乙醛脱氢酶1蛋白家族L1抗体 浓度1mg/ml
yb-5717R Axin 2轴抑制蛋白2抗体 浓度1mg/ml
yb-7027R ANGPTL6血管生成素相关蛋白6抗体 浓度1mg/ml
yb-4687R AMHMuellerian缪勒管激素抑制因子抗体 浓度1mg/ml
yb-7035R EphA8酪氨酸蛋白激酶受体A8抗体 浓度1mg/ml
yb-11383R Acetoacetyl-CoA synthetase脂肪酰辅酶A家族成员1抗体 浓度1mg/ml
yb-11446R ARNTL2芳香烃受体核转录蛋白样2抗体 浓度1mg/ml
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yb-11705R AIP1激活素受体相互作用蛋白1抗体 浓度1mg/ml
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文献和实验相关实验
相关专题 1.为什么要热灭活血清? 加热可以灭活补体系统。激活的补体参与溶解细胞事件，刺激平滑肌收缩，细胞和血小板释放组胺，激活淋巴细胞和巨噬细胞。在进行免疫学 研究、培养ES细胞、昆虫细胞和平滑肌细胞时，推荐使用热灭活血清。 2.L-谷氨酰胺在细胞培养 中重要吗?它在溶液中不稳定吗?L-谷氨酰胺在细胞培养时是重要的。脱掉氨基后，L-谷氨酰胺可作为培养细胞的能量来源、参与蛋白质的合成和核酸代谢。L-谷氨酰胺
条件。在MEM中培养的细胞，很可能在DMEM或M199中同样很容易生长。总之，首选MEM做粘附细胞培养、RPMI-1640做悬浮细胞培养，各种目的无血清培养的首选是AIM V培养基（SFM）。在开始进行新的细胞培养时，可以参考下表所列的条件： 7、为什么要热灭活血清？加热可以灭活补体系统。激活的补体参与溶解细胞事件，刺激平滑肌收缩，细胞和血小板释放组胺，激活淋巴细胞和巨噬细胞。在进行免疫学研究、培养ES细胞、昆虫细胞和平滑肌细胞时，推荐使用热灭活血清。 8、L－谷氨酰胺
1.为什么要热灭活血清？加热可以灭活补体系统。激活的补体参与溶解细胞事件，刺激平滑肌收缩，细胞和血小板释放组胺，激活淋巴细胞和巨噬细胞。在进行免疫学研究、培养ES细胞、昆虫细胞和平滑肌细胞时，推荐使用热灭活血清。2.L－谷氨酰胺在细胞培养中重要吗？它在溶液中不稳定吗？ L－谷氨酰胺在细胞培养时是重要的。脱掉氨基后，L－谷氨酰胺可作为培养细胞的能量来源、参与蛋白质的合成和核酸代谢。L－谷氨酰胺在溶液中经过一段时间后会降解，但是确切的降解率一直没有最终确定。L－谷氨酰胺的降解导致氨的形成，而氨
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组织转谷氨酰胺酶(TGM2)重组蛋白
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