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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
钰博生物
- 检测范围:
见说明书
- 检测方法:
Western blot/Elisa/IHC
- 应用:
科学研究
- 适应物种:
Human/Mouse/Rat
- 标记物:
见说明书
- 样本:
血清液/组织/尿
- 规格:
2 x 96 tests w/plates
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined from mouse blood samples. For most of these tests, plasma or serum samples are used. Data obtained by these investigations provide indications
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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