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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
钰博生物
- 检测范围:
见说明书
- 检测方法:
Western blot/Elisa/IHC
- 应用:
科学研究
- 适应物种:
Human/Mouse/Rat
- 标记物:
见说明书
- 样本:
血清液/组织/尿
- 规格:
50 ug
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文献和实验the opportunity to directly monitor new therapies. In this chapter, we describe a protocol to image, the in vivo distribution of the integrin β7 , expressed by lymphocytes recruited to and retained by the inflamed gut, using a radiolabeled whole antibody
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
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