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- hz082Ra01
- 2025年07月15日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Carbonic Anhydrase VA (CA5A)
- 库存：
100
- 供应商：
上海沪震
碳酸酐酶ⅤA(CA5A)重组蛋白 Recombinant Carbonic Anhydrase VA (CA5A)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No. RPD082Ra01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 24.9kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
碳酸酐酶ⅤA(CA5A)重组蛋白Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
Residues Tyr12~Leu219 (Accession # P43165) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-YKPLAILRH MGPLCATRPQ HWRFQHSYAE KHSNCARHPL WTGPVSSPGG TQQSPINIQW TDSVYDPKLA PLRVSYDAAS CRYLWNTGYF FQVEFDDSCE ESGISGGPLG NHYRLKQFHF HWGATDEWGS EHMVDGHAYP AELHLVHWNS MKYENYKKAT TGENGLAVIG VFLKLGAHHE ALQRLVDILP EVRHKDTQVT MGPFDPSCL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
碳酸酐酶ⅤA(CA5A)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-5462R phospho-c-Jun(Thr249)磷酸化原癌基因c-Jun抗体
hz-15590R CYFRA21-1 肿瘤标志物CYFRA21-1抗体
hz-5458R phospho-c-Jun(Thr91)磷酸化原癌基因c-Jun抗体
hz-5739R Cyclin A1周期素A1蛋白抗体
hz-5743R CDKN3细胞周期蛋白依赖性激酶抑制蛋白3抗体
hz-11187R CK42细胞角蛋白42
hz-4609R CD105CD105抗体
hz-2322R Caspase-7半胱胺酸蛋白酶蛋白-7抗体
hz-4602R CD8CD8/CD8-β抗体
hz-5744R CABLES2细胞周期蛋白依赖性激酶CABLES2抗体
hz-0432R Chicken IgY兔抗鸡IgY抗体
hz-15122R C20orf12820号染色体开放阅读框128抗体
hz-4562R CYP2E1细胞色素P450ⅡE1抗体
hz-4509R CXCL13/BCA1B-淋巴细胞趋化因子抗体
hz-5740R Cdc23细胞分裂周期蛋白23抗体
hz-5738R CKAP5结肠和肝肿瘤高表达蛋白抗体
hz-5745R CKS1细胞周期蛋白依赖性激酶调节亚基1抗体
hz-4614R ICAM1细胞间粘附分子-1抗体
hz-4615R ICAM1细胞间粘附分子1抗体
hz-4616R ICAM1细胞间粘附分子-1抗体
hz-4617R ICAM1细胞间粘附分子-1抗体
hz-4618R ICAM1细胞间粘附分子-1抗体
hz-7624R Cyclophilin F亲环蛋白（亲环素）PPIF抗体
hz-6874R Catalase过氧化氢酶抗体
hz-7098R CLIC4细胞内氯离子通道蛋白4抗体
hz-6373R CGR19细胞生长调控蛋白19/环指蛋白197抗体
hz-7102R CCDC139卷曲螺旋结构域蛋白139抗体
hz-6375R CHMP1A染色体修饰蛋白1抗体（金属蛋白酶1）
hz-11812R CAPS1钙依赖分泌激活蛋白1抗体
hz-11814R CBLN1小脑肽1抗体
hz-11815R CBLN2小脑肽2抗体
hz-11816R CBLN4小脑肽4抗体
hz-11817R CCM2脑海绵状血管畸形蛋白2抗体
hz-11818R CELSR2表皮生长因子样蛋白2抗体
hz-11819R CHRDL1脊索生成素样蛋白1抗体
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No. RPD082Ra01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 24.9kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
碳酸酐酶ⅤA(CA5A)重组蛋白Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
Residues Tyr12~Leu219 (Accession # P43165) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-YKPLAILRH MGPLCATRPQ HWRFQHSYAE KHSNCARHPL WTGPVSSPGG TQQSPINIQW TDSVYDPKLA PLRVSYDAAS CRYLWNTGYF FQVEFDDSCE ESGISGGPLG NHYRLKQFHF HWGATDEWGS EHMVDGHAYP AELHLVHWNS MKYENYKKAT TGENGLAVIG VFLKLGAHHE ALQRLVDILP EVRHKDTQVT MGPFDPSCL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
碳酸酐酶ⅤA(CA5A)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-5462R phospho-c-Jun(Thr249)磷酸化原癌基因c-Jun抗体
hz-15590R CYFRA21-1 肿瘤标志物CYFRA21-1抗体
hz-5458R phospho-c-Jun(Thr91)磷酸化原癌基因c-Jun抗体
hz-5739R Cyclin A1周期素A1蛋白抗体
hz-5743R CDKN3细胞周期蛋白依赖性激酶抑制蛋白3抗体
hz-11187R CK42细胞角蛋白42
hz-4609R CD105CD105抗体
hz-2322R Caspase-7半胱胺酸蛋白酶蛋白-7抗体
hz-4602R CD8CD8/CD8-β抗体
hz-5744R CABLES2细胞周期蛋白依赖性激酶CABLES2抗体
hz-0432R Chicken IgY兔抗鸡IgY抗体
hz-15122R C20orf12820号染色体开放阅读框128抗体
hz-4562R CYP2E1细胞色素P450ⅡE1抗体
hz-4509R CXCL13/BCA1B-淋巴细胞趋化因子抗体
hz-5740R Cdc23细胞分裂周期蛋白23抗体
hz-5738R CKAP5结肠和肝肿瘤高表达蛋白抗体
hz-5745R CKS1细胞周期蛋白依赖性激酶调节亚基1抗体
hz-4614R ICAM1细胞间粘附分子-1抗体
hz-4615R ICAM1细胞间粘附分子1抗体
hz-4616R ICAM1细胞间粘附分子-1抗体
hz-4617R ICAM1细胞间粘附分子-1抗体
hz-4618R ICAM1细胞间粘附分子-1抗体
hz-7624R Cyclophilin F亲环蛋白（亲环素）PPIF抗体
hz-6874R Catalase过氧化氢酶抗体
hz-7098R CLIC4细胞内氯离子通道蛋白4抗体
hz-6373R CGR19细胞生长调控蛋白19/环指蛋白197抗体
hz-7102R CCDC139卷曲螺旋结构域蛋白139抗体
hz-6375R CHMP1A染色体修饰蛋白1抗体（金属蛋白酶1）
hz-11812R CAPS1钙依赖分泌激活蛋白1抗体
hz-11814R CBLN1小脑肽1抗体
hz-11815R CBLN2小脑肽2抗体
hz-11816R CBLN4小脑肽4抗体
hz-11817R CCM2脑海绵状血管畸形蛋白2抗体
hz-11818R CELSR2表皮生长因子样蛋白2抗体
hz-11819R CHRDL1脊索生成素样蛋白1抗体
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文献和实验相关实验
及0-2 mol/L 硫酸铵中的稳定性； c、测定样品在0-50%乙醇、甲醇中的稳定性； d、测定样品在4-40℃的稳定性； e、室温下静置过夜，测定对蛋白水解酶的稳定性。 （2）样品预处理 a、去除样品中颗粒物（0.45-0.22微米膜过滤或者10000 g离心15分钟） b、去除样品中脂类（ 10000 g离心15分钟或有机溶剂抽提） c、去除样品中核酸（加入核酸酶消化或者使核酸沉淀） d、抑制样品中蛋白水解酶（加入蛋白酶抑制剂、低温下快速第一步分离或在蛋白酶缺陷宿主中表达重组蛋白
条件下较高的本底表达，为了让表达系统严谨调控产物表达，能过量表达lacI阻遏蛋白的lacIq 突变菌株常被选为Lac/Tac/trc表达系统的表达菌株。现在的Lac/Tac/trc载体上通常还带有lacIq 基因，以表达更多lacI阻遏蛋白实现严谨的诱导调控。IPTG广泛用于诱导表达系统，但是IPTG有一定毒性，有人认为在制备医疗目的的重组蛋白并不合适，因而也有用乳糖代替IPTG作为诱导物的研究。另外一种研究方向是用lacI的温度敏感突变体，30度下抑制转录，42度开发。热诱导不用添加外来的诱导物，成本
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碳酸酐酶ⅤA(CA5A)重组蛋白
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