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- 沪震生物
- 国产/进口
- hz482Hu02
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Amylase Alpha 1, Salivary (AMY1)
- 库存：
100
- 供应商：
上海沪震
唾液淀粉酶α1(AMY1)重组蛋白 Recombinant Amylase Alpha 1, Salivary (AMY1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB482Hu02
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 57.2kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
唾液淀粉酶α1(AMY1)重组蛋白Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
Residues Ala15~Leu511 (Accession # P04745) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S-AQYSSNTQQ GRTSIVHLFE WRWVDIALEC ERYLAPKGFG GVQVSPPNEN VAIHNPFRPW WERYQPVSYK LCTRSGNEDE FRNMVTRCNN VGVRIYVDAV INHMCGNAVS AGTSSTCGSY FNPGSRDFPA VPYSGWDFND GKCKTGSGDI ENYNDATQVR DCRLSGLLDL ALGKDYVRSK IAEYMNHLID IGVAGFRIDA SKHMWPGDIK AILDKLHNLN SNWFPEGSKP FIYQEVIDLG GEPIKSSDYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGFMP SDRALVFVDN HDNQRGHGAG GASILTFWDA RLYKMAVGFM LAHPYGFTRV MSSYRWPRYF ENGKDVNDWV GPPNDNGVTK EVTINPDTTC GNDWVCEHRW RQIRNMVNFR NVVDGQPFTN WYDNGSNQVA FGRGNRGFIV FNNDDWTFSL TLQTGLPAGT YCDVISGDKI NGNCTGIKIY VSDDGKAHFS ISNSAEDPFI AIHAESKL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
唾液淀粉酶α1(AMY1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-9489R C9ORF469号染色体开放阅读框46抗体
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hz-9488R C9orf869号染色体开放阅读框86抗体
hz-11073R CDH18钙粘附分子18抗体
hz-11019R CD276CD276抗体
hz-9988R C21orf2521号染色体开放阅读框25抗体
hz-9986R C22orf3622号染色体开放阅读框36抗体
hz-11045R C1QL1补体组分1q子成分样蛋白1抗体
hz-4916R CD44CD44抗体
hz-9494R C9orf1149号染色体开放阅读框114抗体
hz-4914R CD8BCD8β链（CD8-β）抗体
hz-9533R C6orf16号染色体开放阅读框1抗体
hz-9585R gamma crystallin Sγ晶状体蛋白S/γS-crystallin抗体
hz-8595R C9orf729号染色体开放阅读框72抗体
hz-11169R Cadherin 10钙粘附分子10抗体
hz-11196R R CadherinR Cadherin/CDH4
hz-4649R Capsid protein VP1大鼠细小病毒H-1株(H-1)抗体
hz-9987R C22orf922号染色体开放阅读框9抗体
hz-9971R CREPT肿瘤高表达细胞周期相关蛋白抗体
hz-9495R C9orf1179号染色体开放阅读框117抗体
hz-9534R C6orf1206号染色体开放阅读框120抗体
hz-11336R C1Q补体C1qα链多肽抗体
hz-11337R C1QC补体C1qγ链多肽抗体
hz-11338R CASK钙/钙调蛋白依赖性丝氨酸蛋白激酶抗体
hz-11339R CLSTN2老年痴呆相关类钙粘蛋白CS2抗体
hz-11340R CPEB3细胞质多聚腺苷酸结合蛋白3抗体
hz-11341R CPLX1Complexin I/复合素1抗体
hz-11342R CPLX2Complexin II/复合素2抗体
hz-11188R CEBP gamma转录调节因子C/EBPγ抗体
hz-11314R CLIP2细胞质连接蛋白2抗体
hz-4869R CD20CD20抗体
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB482Hu02
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 57.2kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
唾液淀粉酶α1(AMY1)重组蛋白Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
Residues Ala15~Leu511 (Accession # P04745) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG S-AQYSSNTQQ GRTSIVHLFE WRWVDIALEC ERYLAPKGFG GVQVSPPNEN VAIHNPFRPW WERYQPVSYK LCTRSGNEDE FRNMVTRCNN VGVRIYVDAV INHMCGNAVS AGTSSTCGSY FNPGSRDFPA VPYSGWDFND GKCKTGSGDI ENYNDATQVR DCRLSGLLDL ALGKDYVRSK IAEYMNHLID IGVAGFRIDA SKHMWPGDIK AILDKLHNLN SNWFPEGSKP FIYQEVIDLG GEPIKSSDYF GNGRVTEFKY GAKLGTVIRK WNGEKMSYLK NWGEGWGFMP SDRALVFVDN HDNQRGHGAG GASILTFWDA RLYKMAVGFM LAHPYGFTRV MSSYRWPRYF ENGKDVNDWV GPPNDNGVTK EVTINPDTTC GNDWVCEHRW RQIRNMVNFR NVVDGQPFTN WYDNGSNQVA FGRGNRGFIV FNNDDWTFSL TLQTGLPAGT YCDVISGDKI NGNCTGIKIY VSDDGKAHFS ISNSAEDPFI AIHAESKL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
唾液淀粉酶α1(AMY1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-9489R C9ORF469号染色体开放阅读框46抗体
hz-9691R C20orf10320号染色体开放阅读框103抗体
hz-9488R C9orf869号染色体开放阅读框86抗体
hz-11073R CDH18钙粘附分子18抗体
hz-11019R CD276CD276抗体
hz-9988R C21orf2521号染色体开放阅读框25抗体
hz-9986R C22orf3622号染色体开放阅读框36抗体
hz-11045R C1QL1补体组分1q子成分样蛋白1抗体
hz-4916R CD44CD44抗体
hz-9494R C9orf1149号染色体开放阅读框114抗体
hz-4914R CD8BCD8β链（CD8-β）抗体
hz-9533R C6orf16号染色体开放阅读框1抗体
hz-9585R gamma crystallin Sγ晶状体蛋白S/γS-crystallin抗体
hz-8595R C9orf729号染色体开放阅读框72抗体
hz-11169R Cadherin 10钙粘附分子10抗体
hz-11196R R CadherinR Cadherin/CDH4
hz-4649R Capsid protein VP1大鼠细小病毒H-1株(H-1)抗体
hz-9987R C22orf922号染色体开放阅读框9抗体
hz-9971R CREPT肿瘤高表达细胞周期相关蛋白抗体
hz-9495R C9orf1179号染色体开放阅读框117抗体
hz-9534R C6orf1206号染色体开放阅读框120抗体
hz-11336R C1Q补体C1qα链多肽抗体
hz-11337R C1QC补体C1qγ链多肽抗体
hz-11338R CASK钙/钙调蛋白依赖性丝氨酸蛋白激酶抗体
hz-11339R CLSTN2老年痴呆相关类钙粘蛋白CS2抗体
hz-11340R CPEB3细胞质多聚腺苷酸结合蛋白3抗体
hz-11341R CPLX1Complexin I/复合素1抗体
hz-11342R CPLX2Complexin II/复合素2抗体
hz-11188R CEBP gamma转录调节因子C/EBPγ抗体
hz-11314R CLIP2细胞质连接蛋白2抗体
hz-4869R CD20CD20抗体
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文献和实验相关实验
内源性FSTL1 通过激活钠‐钾泵引起膜超极化调节痛觉信息传递
分泌到胞外，NKA是一种跨膜蛋白。因此推断，FSTL1 可能是与NKA的胞外部分结合。研究者合成了NKA α1 亚基在胞外的肽段，用BIAcore 分析这些肽段与FSTL1 的结合能力，发现M3M4 和M7M8 肽段与FSTL1 的结合能力较强。进一步的细胞实验发现M3M4 和M7M8 部位有突变的α1 亚基与FSTL1 结合能力较弱，从另一个侧面证明了以下结论：FSTL1 正是通过与M3M4 和M7M8 胞外肽段与NKA α1 亚基结合。 而在培养的DRG 神经元中加入FSTL1 重组蛋白
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唾液淀粉酶α1(AMY1)重组蛋白
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