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- 沪震生物
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- hz073Mu01
- 2025年07月15日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Carbonic Anhydrase VI (CA6)
- 库存：
100
- 供应商：
上海沪震
碳酸酐酶Ⅵ(CA6)重组蛋白 Recombinant Carbonic Anhydrase VI (CA6)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPD073Mu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 19.1kDa
Predicted isoelectric point 5.0
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
碳酸酐酶Ⅵ(CA6)重组蛋白
Subcellular Location Secreted
Residues Asp27~Ser181 (Accession # P18761) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-DGVG ESQWSEQYPS CGGERQSPID VKTEEVMFNP SLKPLSLVNY EKENLEFTMT NNGHTVSIDL PPSMYLETSD GTEFISKAFH FHWGGRDWEL SGSEHTIDGI RSIMEAHFVH FNKEYGTYEN AKDQKNGLAV LAVLFKIDEY AENTYYSDII S
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
碳酸酐酶Ⅵ(CA6)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-3172R phospho-c-Jun (Ser73)磷酸化原癌基因c-Jun抗体
hz-3173R phospho-c-Jun(Ser243)磷酸化原癌基因c-Jun抗体
hz-15079R C1orf861号染色体开放阅读框86抗体
hz-15298R C8ORF598号染色体开放阅读框59抗体
hz-13816R phospho-C5R1 (Ser338)磷酸化补体成分5受体1抗体
hz-13817R Complement component C6补体C6抗体
hz-13818R CEACAM7癌胚抗原相关细胞粘附分子7抗体
hz-13819R Phospho-CEBP beta (Thr188)磷酸化转录调节因子CEBP β抗体
hz-13820R Phospho-CEBP beta (Thr188 + Thr235)磷酸化转录调节因子CEBP β抗体
hz-13821R CEBP Delta转录调节因子C/EBPδ抗体
hz-13822R CEBPE转录调节因子C/EBPε抗体
hz-13823R phospho-CEBPE (Thr74)磷酸化转录调节因子C/EBPε抗体
hz-13826R CEECAM1脑血管内皮细胞粘附分子1抗体
hz-13804R C1QL4补体C1QL4链多肽抗体
hz-13781R C19orf219号染色体开放阅读框2抗体
hz-13807R CCDC62卷曲螺旋结构域蛋白62抗体
hz-13808R CCDC64卷曲螺旋结构域蛋白64抗体
hz-13811R CCDC72卷曲螺旋结构域蛋白72抗体
hz-13812R CCDC73卷曲螺旋结构域蛋白73抗体
hz-13813R CCDC74B卷曲螺旋结构域蛋白74B抗体
hz-13782R C19orf2519号染色体开放阅读框25抗体
hz-13783R C19orf4219号染色体开放阅读框42抗体
hz-13784R C19orf4419号染色体开放阅读框44抗体
hz-13785R C19orf4819号染色体开放阅读框48抗体
hz-13786R C19orf5019号染色体开放阅读框50抗体
hz-13787R C19orf5119号染色体开放阅读框51抗体
hz-13788R C19orf5219号染色体开放阅读框52抗体
hz-13789R C19orf5519号染色体开放阅读框55抗体
hz-13790R C19orf5619号染色体开放阅读框56抗体
hz-13791R C19orf5719号染色体开放阅读框57抗体
hz-13792R C19orf6119号染色体开放阅读框61抗体
hz-13798R C19orf7719号染色体开放阅读框77抗体
hz-13800R C1D核DNA结合蛋白C1D抗体
hz-13757R C1orf1581号染色体开放阅读框58抗体
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPD073Mu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 19.1kDa
Predicted isoelectric point 5.0
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
碳酸酐酶Ⅵ(CA6)重组蛋白
Subcellular Location Secreted
Residues Asp27~Ser181 (Accession # P18761) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-DGVG ESQWSEQYPS CGGERQSPID VKTEEVMFNP SLKPLSLVNY EKENLEFTMT NNGHTVSIDL PPSMYLETSD GTEFISKAFH FHWGGRDWEL SGSEHTIDGI RSIMEAHFVH FNKEYGTYEN AKDQKNGLAV LAVLFKIDEY AENTYYSDII S
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
碳酸酐酶Ⅵ(CA6)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.hz-3172R phospho-c-Jun (Ser73)磷酸化原癌基因c-Jun抗体
hz-3173R phospho-c-Jun(Ser243)磷酸化原癌基因c-Jun抗体
hz-15079R C1orf861号染色体开放阅读框86抗体
hz-15298R C8ORF598号染色体开放阅读框59抗体
hz-13816R phospho-C5R1 (Ser338)磷酸化补体成分5受体1抗体
hz-13817R Complement component C6补体C6抗体
hz-13818R CEACAM7癌胚抗原相关细胞粘附分子7抗体
hz-13819R Phospho-CEBP beta (Thr188)磷酸化转录调节因子CEBP β抗体
hz-13820R Phospho-CEBP beta (Thr188 + Thr235)磷酸化转录调节因子CEBP β抗体
hz-13821R CEBP Delta转录调节因子C/EBPδ抗体
hz-13822R CEBPE转录调节因子C/EBPε抗体
hz-13823R phospho-CEBPE (Thr74)磷酸化转录调节因子C/EBPε抗体
hz-13826R CEECAM1脑血管内皮细胞粘附分子1抗体
hz-13804R C1QL4补体C1QL4链多肽抗体
hz-13781R C19orf219号染色体开放阅读框2抗体
hz-13807R CCDC62卷曲螺旋结构域蛋白62抗体
hz-13808R CCDC64卷曲螺旋结构域蛋白64抗体
hz-13811R CCDC72卷曲螺旋结构域蛋白72抗体
hz-13812R CCDC73卷曲螺旋结构域蛋白73抗体
hz-13813R CCDC74B卷曲螺旋结构域蛋白74B抗体
hz-13782R C19orf2519号染色体开放阅读框25抗体
hz-13783R C19orf4219号染色体开放阅读框42抗体
hz-13784R C19orf4419号染色体开放阅读框44抗体
hz-13785R C19orf4819号染色体开放阅读框48抗体
hz-13786R C19orf5019号染色体开放阅读框50抗体
hz-13787R C19orf5119号染色体开放阅读框51抗体
hz-13788R C19orf5219号染色体开放阅读框52抗体
hz-13789R C19orf5519号染色体开放阅读框55抗体
hz-13790R C19orf5619号染色体开放阅读框56抗体
hz-13791R C19orf5719号染色体开放阅读框57抗体
hz-13792R C19orf6119号染色体开放阅读框61抗体
hz-13798R C19orf7719号染色体开放阅读框77抗体
hz-13800R C1D核DNA结合蛋白C1D抗体
hz-13757R C1orf1581号染色体开放阅读框58抗体
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文献和实验相关实验
及0-2 mol/L 硫酸铵中的稳定性； c、测定样品在0-50%乙醇、甲醇中的稳定性； d、测定样品在4-40℃的稳定性； e、室温下静置过夜，测定对蛋白水解酶的稳定性。 （2）样品预处理 a、去除样品中颗粒物（0.45-0.22微米膜过滤或者10000 g离心15分钟） b、去除样品中脂类（ 10000 g离心15分钟或有机溶剂抽提） c、去除样品中核酸（加入核酸酶消化或者使核酸沉淀） d、抑制样品中蛋白水解酶（加入蛋白酶抑制剂、低温下快速第一步分离或在蛋白酶缺陷宿主中表达重组蛋白
分营养成分可以通过化学成分明确的营养物质如氨基酸维生素等成分组合取代。低血清培养基营养成分大优于基础培养基，仅需添加1％～5％新生牛血清，而对细胞生长、增殖、形态、活性和功能没有影响甚至有所改善。 在国外低血清培养基早已有之，如Gibco等。但是他们的低血清培养基是在基础培养基中选择性的加入重组胰岛素（recombinant insulin）、人源转铁蛋白（human (holo) tran- sferrin）以及牛血清白蛋白等，有些还包含一些生长因子，这些重组蛋白和动物来源成分
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碳酸酐酶Ⅵ(CA6)重组蛋白
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