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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
Type II collagen, the most abundant protein of cartilage matrix, is synthesized as a procollagen molecule including the N-(PIINP) and C-(PIICP) propeptides at each end. Type II procollagen is produced in two forms as the result of alternative
3. Stage II - Setting up the 3D matrix with embedded fibroblasts (allow to contract for 8 days). 1) Assemble the mix using cold reagents at 4°C in a pre-chilled bottle. Keep collagen I on ice. 2) For one T75 flask of confluent
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