重组人泛素，GST标签

产品英文名称：
Recombinant Human Ubiquitin Gly76 to Ala76 mutation

英文简称:
Ubiquitin G76A

中文名称:
重组人泛素G76A突变

背景资料:
The conserved 76 amino acid protein ubiquitin （Ub） regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins. Ubiquitination occurs through sequential steps catalyzed by activating （E1）， conjugating （E2）， and ligase （E3） enzymes. The final step results in the formation of an isopeptide bond betwRecombinant human ubiquitin featuring a Gly76 to Ala76 mutation that， by inhibiting the ubiquitin hydrolases， prevents the removal of ubiquitin from protein ubiquitin conjugates. Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography.n Ub’s C-terminal glycine residue （G76） and a lysine residue of the target protein， although N-terminal ubiquitination is also known. Outcomes of this modification include destabilization of the conjugated protein， altered protein tra大肠杆菌中重组表达icking and functional modulation. After targeting the protein for specific localizations， ubiquitin is released from the substrate by deubiquitinating enzymes. A mutant ubiquitin， having a Gly to Ala substitution at the C-terminus （G76A ubiquitin） supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process. As consequence， the Ub derivative becomes irreversibly conjugated to protein， shifting the equilibrium betwRecombinant human ubiquitin featuring a Gly76 to Ala76 mutation that， by inhibiting the ubiquitin hydrolases， prevents the removal of ubiquitin from protein ubiquitin conjugates. Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography.n the bound and unbound form in the direction of conjugation， at the expense of the frRecombinant human ubiquitin featuring a Gly76 to Ala76 mutation that， by inhibiting the ubiquitin hydrolases， prevents the removal of ubiquitin from protein ubiquitin conjugates. Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography. form.

产品描述:
Recombinant human ubiquitin featuring a Gly76 to Ala76 mutation that， by inhibiting the ubiquitin hydrolases， prevents the removal of ubiquitin from protein ubiquitin conjugates. Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography.
来源宿主:
大肠杆菌中重组表达

序列:


溶解建议:
Sterile Filtered clear solution. Diluted in PBS plus 5% glycerol.

保存建议：
Store vial at -20℃ to -80℃. When stored at the recommended temperature， this protein is stable for 12 months. Please prevent freeze-thaw cycles.
其他简介：

  ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。