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DNA extraction for 8-OHdG assay
for DNA extraction from human & animal parenchyma tissue
DNA Extractor(r) TIS Kit is specially designed for the extraction of DNA from human and animal parenchyma tissue. The base of this kit is a procedure using Sodium Iodide (NaI) as a chaotropic agent, which allows for a safe extraction without hazardous phenol/chloroform. The procedure using Sodium Iodide is an extraction procedure which causes less oxidation of DNA. Additionally, the DNA Extractor(r) TIS Kit is able to inhibit the oxidation of DNA by using an Oxidation Inhibitor. It is the most useful DNA extraction kit for the assay of 8-OHdG (8-Hydroxy-2'-deoxyguanosine), which is a marker of oxidative stress.
[Features]
- Useful for detection and determination of 8-OHdG, a marker of oxidative
stress. - Achieve inhibition of the further DNA oxidation by using an oxidation inhibitor
- Suitable for a wide range of applications, which require DNA extraction from human and animal parenchyma tissues
[Kit Contents] ( 50 Reactions) (uL: microlitter)
- Lysis Solution 2 bottles x 75 mL
- Enzyme Reaction Solution 1 bottle x 15 mL
- RNase Solution 1 vial x 50 uL
- Protein Digestion Solution 1 vial x 750 uL
- Oxidation Inhibitor 1 vial x 350 uL
- Sodium Iodide Solution 1 bottle x 15 mL
- Alcohol Solution 1 bottle x 30 mL
- PEG Solution 1 bottle x 20 mL
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文献和实验Vybrant® DyeCycle Green and Orange Stains
material is supplied for approximately 200 fl ow cytometry assays based on a 1 mL test volume. Approximate fluorescence excitation/emission maxima: Vybrant® DyeCycle™ Green stain: 506/534 nm, bound to DNA; Vybrant® DyeCycle™ Orange stain: 519/563 nm
实验试剂 Dynal® magnet: see www.invitrogen.com/magnets-selection. Sterile, round-bottomed 2 ml microcentrifuge tubes. Micropipettors and sterile, disposable pipette tips. 65°C water-bath or heating block
Extraction of DNA using DNAzol® Reagent
pipette tips by cutting 2-3 mm from the ends of plastic pipette tips. Mix DNA solutions by inversion; avoid shaking or use of a Vortex for mixing. Homogenization of tissues: Homogenize tissue samples in a hand-held glass/Teflon® homogenizer







