- ¥1013
- promega
- 进口
- Z6010
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
10 preps
| Z6010 | ReliaPrep™ RNA Cell Miniprep System | 10 preps | 1,013 |
| Z6011 | ReliaPrep™ RNA Cell Miniprep System | 50 preps | 2,834 |
| Z6012 | ReliaPrep™ RNA Cell Miniprep System | 250 preps | 12,958 |
| Z6110 | ReliaPrep™ RNA Tissue Miniprep System | 10 preps | 3,134 |
| Z6111 | ReliaPrep™ RNA Tissue Miniprep System | 50 preps | 4,742 |
| Z6112 | ReliaPrep™ RNA Tissue Miniprep System | 250preps | 18,201 |
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
cell pellet in 100 μl of GTE buffer (50 mM Glucose, 25 mM Tris-Cl, 10 mM EDTA, pH 8). Vortex gently if necessary. 4. Add 200 μl of NaOH/SDS lysis solution (0.2 M NaOH, 1% SDS). Invert tube 6-8 times. 5. IMMEDIATELY add 150 μl
plus adhered phage to 10ml of T-broth (one liter contains 10g bactotryptone, 5g NaCl) containing 10mM Mg++ . Shake at 37℃ for 3-6hrs, until cell lysis. In the meantime, check to see that the cultures first become turbid before they clear. That is, the bacterial
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