R&D Systems货号KGE002B现货cAMP Par
ameter Assay Kit上海睿安生物R&D Systems-Novus-Tocris官方正式授权代理19121878899- ￥5207.01
- R&D Systems
- made in USA
- KGE002B
- 2026年02月05日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
cAMP Parameter Assay Kit
- 保质期：
1年
- 保存条件：
-20°C
- 库存：
10⁺盒
- 供应商：
上海睿安生物19121878899
- 规格：
1Kit/盒
R&D Systems货号KGE002B现货cAMP Parameter Assay Kit上海睿安生物R&D Systems-Novus-Tocris官方正式授权代理19121878899
Key Product Details
Assay Length:3.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates(50µl),Cell Lysates(100µl),Serum(20µl),EDTA Plasma(20µl),Heparin Plasma(20µl),Saliva(50µl), Urine(10µl)
Sensitivity:8.57 pmol/ml
Assay Range:3.75-240 pmol/ml(Cell Culture Supernates,Cell Lysates,Serum,EDTA Plasma,Heparin Plasma,Saliva,Urine)
Product Summary for cAMP Parameter Assay Kit
Product Specifications
Measurement:Quantitative ELISA
Detection Method:Colorimetric-450nm(TMB)
Conjugate:Biotin
Species:Multi-Species
Specificity:Measures cyclic AMP levels in various samples
Cross-reactivity:Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference:No significant interference observed with available related molecules.
精度
Intra-Assay Precision(Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision(Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping:The product is shipped with dry ice or equivalent.Upon receipt,store it immediately at the temperature recommended below.
Stability & Storage:Store the unopened product at -20 to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background:cAMP
cAMP (adenosine 3’5’-cyclic monophosphate) is a ubiquitous intracellular second messenger that mediates a diverse range of cellular processes in all organisms from bacteria to higher eukaryotes. It is converted from adenosine triphosphate (ATP) by adenylyl cyclases (ACs), and is inactivated by phosphodiesterases (PDEs) which catalyze its hydrolysis to 5’-AMP. In mammals, upon interaction with extracellular ligands, G protein coupled receptors (GPCRs) linked to Gαs activate the family of nine transmembrane ACs to increase intracellular cAMP. In contrast, GPCRs associated with Gαi/o inhibit the synthesis of cAMP by transmembrane ACs. cAMP binds and activates protein kinase A (PKA), the guanine nucleotide exchange factors Epac1/2, and cyclic nucleotide-gated ion channels. It can be released extracellularly where it is converted to adenosine by phosphodiesterases and nucleotidases.
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文献和实验R&D Systems货号KGE002B现货cAMP Parameter Assay Kit上海睿安生物R&D Systems-Novus-Tocris官方正式授权代理19121878899
Key Product Details
Assay Length:3.5 hours
Sample Type & Volume Required Per Well:Cell Culture Supernates(50µl),Cell Lysates(100µl),Serum(20µl),EDTA Plasma(20µl),Heparin Plasma(20µl),Saliva(50µl), Urine(10µl)
Sensitivity:8.57 pmol/ml
Assay Range:3.75-240 pmol/ml(Cell Culture Supernates,Cell Lysates,Serum,EDTA Plasma,Heparin Plasma,Saliva,Urine)
Product Summary for cAMP Parameter Assay Kit
Product Specifications
Measurement:Quantitative ELISA
Detection Method:Colorimetric-450nm(TMB)
Conjugate:Biotin
Species:Multi-Species
Specificity:Measures cyclic AMP levels in various samples
Cross-reactivity:Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference:No significant interference observed with available related molecules.
精度
Intra-Assay Precision(Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision(Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with the appropriate diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping:The product is shipped with dry ice or equivalent.Upon receipt,store it immediately at the temperature recommended below.
Stability & Storage:Store the unopened product at -20 to -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background:cAMP
cAMP (adenosine 3’5’-cyclic monophosphate) is a ubiquitous intracellular second messenger that mediates a diverse range of cellular processes in all organisms from bacteria to higher eukaryotes. It is converted from adenosine triphosphate (ATP) by adenylyl cyclases (ACs), and is inactivated by phosphodiesterases (PDEs) which catalyze its hydrolysis to 5’-AMP. In mammals, upon interaction with extracellular ligands, G protein coupled receptors (GPCRs) linked to Gαs activate the family of nine transmembrane ACs to increase intracellular cAMP. In contrast, GPCRs associated with Gαi/o inhibit the synthesis of cAMP by transmembrane ACs. cAMP binds and activates protein kinase A (PKA), the guanine nucleotide exchange factors Epac1/2, and cyclic nucleotide-gated ion channels. It can be released extracellularly where it is converted to adenosine by phosphodiesterases and nucleotidases.
Overview of Biacore Systems and Their Applications
. Bokken, G.C., Corbee, R.J., van Knapen, F., and Bergwerff, A.A. 2003. Immunochemical detection of Salmonella group B, D and E using an optical surface plasmon resonance biosensor. FEMS Microbiol. Lett. 222:75
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
： A、蛋白芯片组成 • 4 张芯片（也有个别芯片是8张的）。4张芯片完全一样，每张芯片上每个被检测的指标都有复孔做对照。也包括的阳性对照、阴性对照组。 • 芯片缓冲液 • 洗液 - 25X • 生物素 或 HRP-偶联检测抗体 • 4-巢小盒 • 透明模板Transparency Overlay Template • 产品说明书 B、蛋白芯片的结构 C、检测样本类别 细胞上清液，细胞溶解产物，血清，血浆等。 D、保存条件与稳定
