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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
+4C
- 英文名:
BIOLUMINEX Hand spot kit with X-ray film (DISCONTINUED)
- 供应商:
上海汇谱仪器有限公司
- 规格:
1 Kit
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文献和实验Whatman #1 filter paper cut to size of X-ray film with corners numbered with 14 C-ink Protocol: Open Kapak and transfer solution to 32 P liquid waste. Pipet 10-20 ml of 65℃ 5x SSPE into the bag, gently mix, add this solution to liquid waste. Transfer
Screening Colonies by Hybridization with Radiolabeled Probe
then are prehybridized to block binding sites, and the radiolabeled probe added thereafter will compete for specific binding sites. After washing, the air died filters are exposed to an X-ray film which shows positive colonies.. Supplies & Equipment:
Analys of Genomic DNA by Southe
. Do not expect a signal from single copy genes. (1 to 6 days) After washing, blot the membrane with filter paper (Whatman 3MM or S&S GB003) to remove most of the excess moisture. Wrap moist blots in plastic wrap prior to autoradiography. Expose to X-ray film
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