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Roche 6366244001 X-tremeGENE H

P DNA转染试剂13611631389上海睿安生物
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  • ￥3015.60
  • Roche
  • Made in Germany
  • 6366244001
  • 2026年03月08日
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    上海睿安生物科技有限公司
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    • 保存条件：

      −20°C

    • 保质期：

      1年

    • 英文名：

      Xtremegene, transfection, transfection reagent

    • 库存：

      100⁺支

    • 供应商：

      上海睿安生物13611631389

    • 规格：

      0.4ml/支

    Roche货号6366244001，X-tremeGENE HP DNA转染试剂13611631389上海睿安生物

     

    Roche货号6366236001，X-tremeGENE HP DNA转染试剂13611631389上海睿安生物

    产品细节图片1

    Roche货号6366546001，X-tremeGENE HP DNA转染试剂13611631389上海睿安生物

    X-tremeGENE™ HP DNA转染试剂

    High-performance polymer reagent for transfecting many cell lines

    说明

    一般描述:X-tremeGENE HP DNA转染试剂是一款多组分试剂，能够与DNA形成复合物，并将其递送至动物或昆虫细胞中。

    特点和优势:X-tremeGENE HP DNA转染试剂是一种高效转染试剂，可成功转染多种真核细胞，包括昆虫细胞和难转染细胞系(如Sf9、U-2 OS、MEF、MCF7、Hep G2、PC-3、HeLa、HT-29、HT-1080、K-562、RAW 264.7、Jurkat、PC-12和HCT 116)。

    • 简单易用的非脂质体试剂 无动物源性成分，室温稳定，经0.2μm滤膜过滤，并且在有血清培养液中仍保持活性。
    • 高转染效率 对于原代细胞和使用其它试剂难转染的肿瘤细胞系有高转染效率。
    • 结果生理相关性好 使用细胞毒性低的试剂。
    • 满足高通量实验 实现简单一致的靶标评估。

    质量

    每一批次的X-tremeGENE HP DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE HP DNA转染试剂(试剂DNA比为3:1μl/μg DNA)向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

    外形:本产品溶于80%乙醇中，经0.2μm滤膜过滤。无人源或动物源成分。次数:使用标准步骤时，在96孔板内，1ml X-tremeGENE™ HP DNA转染试剂可以进行10000次转染。

    其他说明:仅用于生命科学研究。不可用于诊断。

    产品细节图片2产品细节图片3产品细节图片4产品细节图片5

    同行评审论文(部分文献)

    Cosmomycin C inhibits signal transducer and activator of transcription 3 (STAT3) pathways in MDA-MB-468 breast cancer cell.
    Jihoon Kim et al.
    Bioorganic & medicinal chemistry, 19(24), 7582-7589 (2011-11-11)
    The signal transducer and activator of transcription 3 (STAT3) is constitutively activated in cancer cells. Therefore, blocking the aberrant activity of STAT3 in tumor cells is a validated therapeutic strategy. To discover novel inhibitors of STAT3 activity, we screened against
    Positron emission tomography imaging of tumors expressing the human chemokine receptor CXCR4 in mice with the use of 64Cu-AMD3100.
    Ido D Weiss et al.
    Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 14(1), 106-114 (2011-02-25)
    Expression of CXCR4 in cancers has been correlated with poor prognosis and increased metastasis. Quantifying CXCR4 expression non-invasively might aid in prognostication and monitoring therapy. We evaluated a radiolabeled antagonist of CXCR4, ⁶⁴Cu-AMD3100, as a positron-emitting imaging agent. CXCR4-transfected or
    Non-phosphorylated FTY720 induces apoptosis of human microglia by activating SREBP2.
    Takashi Yoshino et al.
    Cellular and molecular neurobiology, 31(7), 1009-1020 (2011-04-27)
    A synthetic analog of sphingosine named FTY720 (Fingolimod), phosphorylated by sphingosine kinase-2, interacts with sphingosine-1-phosphate (S1P) receptors expressed on various cells. FTY720 suppresses the disease activity of multiple sclerosis (MS) chiefly by inhibiting S1P-dependent egress of autoreactive T lymphocytes from
    5-ALA-PDT induces RIP3-dependent necrosis in glioblastoma.
    Isabelle Coupienne et al.
    Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 10(12), 1868-1878 (2011-10-29)
    Glioblastoma constitute the most frequent and deadliest brain tumors of astrocytic origin. They are resistant to all current therapies and are associated with a high rate of recurrence. Glioblastoma were previously shown to respond to treatments by 5-aminolevulinic acid (5-ALA)-based
    Helicobacter pylori upregulates Nanog and Oct4 via Wnt/β-catenin signaling pathway to promote cancer stem cell-like properties in human gastric cancer.
    Xin Yong et al.
    Cancer letters, 374(2), 292-303 (2016-03-05)
    Helicobacter pylori (H. pylori) infection is considered a major risk factor for gastric cancer. CagA behaves as a major bacterial oncoprotein playing a key role in H. pylori-induced tumorigenesis. Cancer stem cells (CSCs) are believed to possess the ability to initiate tumorigenesis

    产品细节图片6

    罗氏在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一，业务范围主要涉及药品、医疗诊断、维生素和精细化工、香精香料等四个领域。

    订购信息:

    货号品名规格
    11873580001Complete, EDTA-free, 20 tablet20 tablets
    4693116001Complete,EASYpack,20 Tab.蛋白酶抑制剂20 tablets (in foil blisters)
    4693132001Complete,EDTA-free,EASYpack,2020 tablets (in foil blisters)
    4693124001Complete,Mini,EASYpack,30 Tab.30 tablets (in foil blisters)
    4906837001PhosStop, 20 Tablets 磷酸酶抑制剂混片20 tablets (each tablet for 10 ml)
    4906845001PhosStop, 10 Tablets 磷酸酶抑制剂混片10 tablets (each tablet for 10 ml)
    10843555001Hygromycin B 潮霉素B1 g (20 ml) sterile-filtered
    11243233001Protein G-Agarose5 ml
    11383221001BCIP, 4-toluidine salt, soluti 4-甲苯胺盐3 ml (150 mg)
    11383213001NBT, Solution50 reactions (sample)
    11096176001Blocking Reagent 封闭剂50 g
    11088866001Collagenase D, 500mg 胶原酶D500 mg
    10107409001Mitomycin C 丝裂méi霉素C2 mg
    10165921001Pronase from Streptomyces gris 链霉蛋白酶1 g (non-sterile)
    12156792910In Situ Cell Death TMR 细胞凋亡，tunel检测方式1 kit (50 tests)
    11685597910Biotin RNA Labeling Mix 10x 生物素核酸标记40 µl (20 reactions)
    11277073910DIG RNA Labeling Mixture, 10x40 µl (20 reactions)
    11093274910Anti-digoxigenin AP-conjungate150 U (200 µl)
    6366244001X-tremeGENE HP DNA Transf. Reag. 0.4 ml 转染试剂0.4 ml
    6366236001X-tremeGENE HP DNA Transf. Reag. 1.0 ml 转染试剂1.0 ml
    4913850001FS Universal SYBR Green Master4 x 1.25 ml (for 200 reactions of 50 µl final reaction volume)
    4913914001FS Universal SYBR Green Master10 x 5 ml (for 2,000 reactions of 50 µl final reaction volume)

    产品细节图片7产品细节图片8
    睿安公司专业技术服务
    荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
    产品细节图片9
    睿安公司代理及优势品牌：
    免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
    耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
    分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
    综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
    自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
    肥胖代谢类：Biovendor、ALPCO
    表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
    其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
    Proteintech 、Socorex 、Vector
    产品细节图片10产品细节图片11产品细节图片12

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    该产品被引用文献

    Roche货号6366244001，X-tremeGENE HP DNA转染试剂13611631389上海睿安生物

     

    Roche货号6366236001，X-tremeGENE HP DNA转染试剂13611631389上海睿安生物

    引用文献图片1

    Roche货号6366546001，X-tremeGENE HP DNA转染试剂13611631389上海睿安生物

    X-tremeGENE™ HP DNA转染试剂

    High-performance polymer reagent for transfecting many cell lines

    说明

    一般描述:X-tremeGENE HP DNA转染试剂是一款多组分试剂，能够与DNA形成复合物，并将其递送至动物或昆虫细胞中。

    特点和优势:X-tremeGENE HP DNA转染试剂是一种高效转染试剂，可成功转染多种真核细胞，包括昆虫细胞和难转染细胞系(如Sf9、U-2 OS、MEF、MCF7、Hep G2、PC-3、HeLa、HT-29、HT-1080、K-562、RAW 264.7、Jurkat、PC-12和HCT 116)。

    • 简单易用的非脂质体试剂 无动物源性成分，室温稳定，经0.2μm滤膜过滤，并且在有血清培养液中仍保持活性。
    • 高转染效率 对于原代细胞和使用其它试剂难转染的肿瘤细胞系有高转染效率。
    • 结果生理相关性好 使用细胞毒性低的试剂。
    • 满足高通量实验 实现简单一致的靶标评估。

    质量

    每一批次的X-tremeGENE HP DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE HP DNA转染试剂(试剂DNA比为3:1μl/μg DNA)向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

    外形:本产品溶于80%乙醇中，经0.2μm滤膜过滤。无人源或动物源成分。次数:使用标准步骤时，在96孔板内，1ml X-tremeGENE™ HP DNA转染试剂可以进行10000次转染。

    其他说明:仅用于生命科学研究。不可用于诊断。

    引用文献图片2引用文献图片3引用文献图片4引用文献图片5

    同行评审论文(部分文献)

    Cosmomycin C inhibits signal transducer and activator of transcription 3 (STAT3) pathways in MDA-MB-468 breast cancer cell.
    Jihoon Kim et al.
    Bioorganic & medicinal chemistry, 19(24), 7582-7589 (2011-11-11)
    The signal transducer and activator of transcription 3 (STAT3) is constitutively activated in cancer cells. Therefore, blocking the aberrant activity of STAT3 in tumor cells is a validated therapeutic strategy. To discover novel inhibitors of STAT3 activity, we screened against
    Positron emission tomography imaging of tumors expressing the human chemokine receptor CXCR4 in mice with the use of 64Cu-AMD3100.
    Ido D Weiss et al.
    Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 14(1), 106-114 (2011-02-25)
    Expression of CXCR4 in cancers has been correlated with poor prognosis and increased metastasis. Quantifying CXCR4 expression non-invasively might aid in prognostication and monitoring therapy. We evaluated a radiolabeled antagonist of CXCR4, ⁶⁴Cu-AMD3100, as a positron-emitting imaging agent. CXCR4-transfected or
    Non-phosphorylated FTY720 induces apoptosis of human microglia by activating SREBP2.
    Takashi Yoshino et al.
    Cellular and molecular neurobiology, 31(7), 1009-1020 (2011-04-27)
    A synthetic analog of sphingosine named FTY720 (Fingolimod), phosphorylated by sphingosine kinase-2, interacts with sphingosine-1-phosphate (S1P) receptors expressed on various cells. FTY720 suppresses the disease activity of multiple sclerosis (MS) chiefly by inhibiting S1P-dependent egress of autoreactive T lymphocytes from
    5-ALA-PDT induces RIP3-dependent necrosis in glioblastoma.
    Isabelle Coupienne et al.
    Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 10(12), 1868-1878 (2011-10-29)
    Glioblastoma constitute the most frequent and deadliest brain tumors of astrocytic origin. They are resistant to all current therapies and are associated with a high rate of recurrence. Glioblastoma were previously shown to respond to treatments by 5-aminolevulinic acid (5-ALA)-based
    Helicobacter pylori upregulates Nanog and Oct4 via Wnt/β-catenin signaling pathway to promote cancer stem cell-like properties in human gastric cancer.
    Xin Yong et al.
    Cancer letters, 374(2), 292-303 (2016-03-05)
    Helicobacter pylori (H. pylori) infection is considered a major risk factor for gastric cancer. CagA behaves as a major bacterial oncoprotein playing a key role in H. pylori-induced tumorigenesis. Cancer stem cells (CSCs) are believed to possess the ability to initiate tumorigenesis

    引用文献图片6

    罗氏在制药和诊断领域是世界领先的以研发为基础，以创新为驱动的健康医疗公司之一，业务范围主要涉及药品、医疗诊断、维生素和精细化工、香精香料等四个领域。

    订购信息:

    货号品名规格
    11873580001Complete, EDTA-free, 20 tablet20 tablets
    4693116001Complete,EASYpack,20 Tab.蛋白酶抑制剂20 tablets (in foil blisters)
    4693132001Complete,EDTA-free,EASYpack,2020 tablets (in foil blisters)
    4693124001Complete,Mini,EASYpack,30 Tab.30 tablets (in foil blisters)
    4906837001PhosStop, 20 Tablets 磷酸酶抑制剂混片20 tablets (each tablet for 10 ml)
    4906845001PhosStop, 10 Tablets 磷酸酶抑制剂混片10 tablets (each tablet for 10 ml)
    10843555001Hygromycin B 潮霉素B1 g (20 ml) sterile-filtered
    11243233001Protein G-Agarose5 ml
    11383221001BCIP, 4-toluidine salt, soluti 4-甲苯胺盐3 ml (150 mg)
    11383213001NBT, Solution50 reactions (sample)
    11096176001Blocking Reagent 封闭剂50 g
    11088866001Collagenase D, 500mg 胶原酶D500 mg
    10107409001Mitomycin C 丝裂méi霉素C2 mg
    10165921001Pronase from Streptomyces gris 链霉蛋白酶1 g (non-sterile)
    12156792910In Situ Cell Death TMR 细胞凋亡，tunel检测方式1 kit (50 tests)
    11685597910Biotin RNA Labeling Mix 10x 生物素核酸标记40 µl (20 reactions)
    11277073910DIG RNA Labeling Mixture, 10x40 µl (20 reactions)
    11093274910Anti-digoxigenin AP-conjungate150 U (200 µl)
    6366244001X-tremeGENE HP DNA Transf. Reag. 0.4 ml 转染试剂0.4 ml
    6366236001X-tremeGENE HP DNA Transf. Reag. 1.0 ml 转染试剂1.0 ml
    4913850001FS Universal SYBR Green Master4 x 1.25 ml (for 200 reactions of 50 µl final reaction volume)
    4913914001FS Universal SYBR Green Master10 x 5 ml (for 2,000 reactions of 50 µl final reaction volume)

    引用文献图片7引用文献图片8
    睿安公司专业技术服务
    荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
    引用文献图片9
    睿安公司代理及优势品牌：
    免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
    耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
    分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
    综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
    自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
    肥胖代谢类：Biovendor、ALPCO
    表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
    其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
    Proteintech 、Socorex 、Vector
    引用文献图片10引用文献图片11引用文献图片12

    相关实验

    • 罗氏公司新推出两款高性能的DNA转染试剂

      相关专题 总有一种转染方法适合你 近日，罗氏公司新推出了两款高性能的DNA转染试剂，为其细胞分析产品线再添新军。这两款名为X-tremeGENE 9和X-tremeGENE HP的转染试剂沿用了X-tremeGENE siRNA 转染试剂的前缀，应该与大名鼎鼎的Fugene 6不属于一类。据罗氏介绍，这两个产品是多组分的独特试剂，并非脂质体。它们能够提供高的转染效率、重复性和细胞存活率。 这两款新产品都融合了罗氏二十

    • RNAse A Treatment of Mouse Cells

      RNAse A � Roche, 109169 Triton X-100 � Eurobio, GAUTTR00-01 Back to top Reviewer Comments Alessia Buscaino , Wellcome Trust (formerly of Akhtar Lab, EMBL). This protocol is also suitable for Drosophila SL-2 cells.

    • Co-Expression of human DNA primase p49-p58 subunits

      Adapted from W.C.Copeland,Protein Exp.& Purif 9,(1997)1-9 and Schneider,et.al.,JBC 273:34 (1998)21608-21615. Obtained from Fanning lab and revised by LSM,7/23/01 Obtained cell stock of BL21(DE3)cells cotransformed with pET21a-Hp49 (ampicillin

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    文献支持
    Roche 6366244001 X-tremeGENE HP DNA转染试剂13611631389上海睿安生物
    ￥3015.60