相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
血液基因组DNA提取(高纯度)
- 库存:
1
- 供应商:
研卉生物
- 规格:
50T
描述
Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.Highlights
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes
• Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
• Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids
Applications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed Protocol
规格
| Sample Type (General): | Blood |
|---|---|
| Downstream Application: | Cloning, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Product Line: | GeneJET |
| Product Size: | 50 preps |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验-HCl, pH8.5。DNA 洗脱溶液,室温密闭贮存。二、简介总RNA 和基因组DNA 同步纯化试剂盒是为从生物样品中同步纯化总RNA 和基因组DNA 设计的,可从≤1×107 动物细胞、100mg 动物组织或5×107 酵母菌中提取多至150μg RNA 和20μg DNA。本试剂盒采用全新的相分离技术分离、纯化RNA 和DNA,结合silica 膜选择性吸附DNA 的原理,达到快速、同步纯化RNA 和基因组DNA 的目的。本试剂盒纯化的总RNA 分子完整、纯度高,适合用于NorthernBlot、RT
以下操作按照天根产品 DP305 植物基因组 DNA 提取试剂盒的说明书进行,所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品,样本量不同可能现象与本文有所差异。实验准备:1. 植物叶片 研钵 液氮2. 无水乙醇 巯基乙醇3. 移液器及配套无菌枪头(10 μl, 200 μl ,1ml),1.5 ml离心管4. 涡旋振荡器,金属浴/水浴,实验准备-试剂盒准备1:使用前先在漂洗液PW和GD中加入无水乙醇,加入体积请参照瓶上的标签。实验准备-试剂盒准备2:准备实验时,将缓冲
yaqingxinlan 请问哪位知道Axygen的DNA甲基化的纯化试剂盒在哪里能买到?怎么联系,非常感谢,急急急急急 honglian198410 推荐你用ZMYO的甲基化试剂盒,我们用的这个比较好,只需要改良一下他的protocol tyssxlth 我用ZMYO的做不出来,用传统的方法做出来了。 贺龙23 请问怎么修改ZYMO公司的甲
技术资料暂无技术资料 索取技术资料








