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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
CD80 (CD80) 抗体 (FITC)
- 规格:
0.1ml/0.2ml
CD80 (CD80) 抗体 (FITC)
anti-CD80 (CD80) antibody (FITC)
抗原
CD80 (CD80)
别名 CD8, Leu2, MAL, p32, B71, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
适用
其他选择 人
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (MEM-233)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术(FACS)
Pubmed 9个引用可参考
规格 100 tests
发货至 中国 (更改)
发货时间 29至35个工作日
产品细节 CD80 (CD80) 抗体 (FITC)
免疫原 CD80 fusion protein
克隆位点 MEM-233
亚型 IgG1
特异性 The antibody reacts with CD80.
目标详细情况 CD80 (CD80) 抗体 (FITC)
别名 CD80
背景 CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
基因ID 941
NCBI登录号 NP_005182
UniProt P33681
研究领域 Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
使用细节 CD80 (CD80) 抗体 (FITC)
应用备注 Flow cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10^6 cells in a suspension.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 CD80 (CD80) 抗体 (FITC)
缓冲液 PBS containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free BSA as a stabilizer
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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文献和实验CD80 分子 CD80 常用单克隆抗体或代号: B7- 1,BB1 主要表达细胞: Ba,Mac,TStr [B] 分子质量(kDa)和结构: gp60(IgSF) 功 能: 活化B细胞抗原,CD28、CTLA- 4配体,提供T细胞共刺激信号 CD80 Aka B7-1, BB1 Ligand for T cell marker CD28 or CTLA4 (CD152
当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation
The following method describes the generation of a recombinant vaccinia virus expressing a costimulatory molecule (human CD80 or B7.1). The procedure first requires the cloning, by classical methods not described here, of the gene
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