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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
Integrin alpha M (ITGAM) 抗体 (FITC)
- 规格:
0.1ml/0.2ml
Integrin alpha M (ITGAM)
别名 CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi
适用
其他选择 人, 猴
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (ICRF44)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术(FACS)
Pubmed 4个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb118515
产品细节 Integrin alpha M (ITGAM) 抗体 (FITC)
免疫原 Rheumatoid synovial cells and human monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
克隆位点 ICRF44
亚型 IgG1
纯化方法 Affinity Chromatography on Protein G.
目标详细情况 Integrin alpha M (ITGAM) 抗体 (FITC)
别名 CD11b
背景 CD11b is a 165kD molecule also known as the alpha M integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
基因ID 3684
NCBI登录号 NP_000623
UniProt P11215
研究领域 Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
使用细节 Integrin alpha M (ITGAM) 抗体 (FITC)
应用备注 Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 Integrin alpha M (ITGAM) 抗体 (FITC)
浓度 0.1 mg/mL
缓冲液 PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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文献和实验光信号强度高于AlexaFluor 405染料1.7倍。绿色(488nm处激发)CF 488A、Alexa Fluor 488、FITC、FAM、DyLight 488、Cy2等----针对488nm 氩离子激光器的绿色荧光染料;以上染料其标记的抗体蛋白适用于所有配备488nm氩离子激光器的流式细胞仪;流式细胞仪的FL1通道检测;或者可用于荧光显微镜技术;CF 488A最低限度的带电量降低了与抗体耦联物的非特异性结合;在红色通道溢出少于Alexa Fluor 488; 耐光性好; 水溶性好 和 pH 不敏
Na2 HPO4 ?12H2 O 2.58g NaH2 PO4 ?2H2 O 0.44g NaCl 8.50g 蒸馏水加至 1000ml (4)Sephadex G-25(或G-50)柱(1.2×30cm) (5)抗体 (6)荧光素:FITC(或TRITC) 【操作方法】 下面介绍直接法和半透膜法。 1、直接法(Marshall法) (1)取适量纯化的抗体,用0.025mol/L pH9.0 CB将抗体溶液的蛋白
0.05% sodium azide (preservative) 0.01M PBS pH7.2 (TBS pH7.6 used in primary antibody dilution buffer produces weaker staining) Note: 1) Antibodies diluted using this buffer can be stored at 4 ºC
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