Integrin alpha M (ITGAM) 抗体

Integrin alpha M (ITGAM) 抗体
抗原
Integrin alpha M (ITGAM)
别名 CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi
适用
其他选择 人, 猴
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (ICRF44)
标记
其他选择 非结合性
Integrin alpha M (ITGAM) 抗体应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）, Functional Studies (Func), 免疫沉淀（IP）
Pubmed 5个引用可参考
规格 0.2 mg
发货至 中国 (更改)
产品细节
免疫原 Rheumatoid synovial cells and Human monocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
克隆位点 ICRF44
亚型 IgG1
特异性 This antibody recognizes the CD11b cell surface glycoprotein. Clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.
无交叉反应 猫
交叉反应 （详细） Species reactivity (tested):Human, Cynomolgus Monkey , Rhesus Monkey, Baboon.
纯化方法 Affinity Chromatography on Protein G
目标详细情况
别名 CD11b
背景 CD11b is a 165 kD molecule also know as the alpha-M integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta-2 integrin and is found on monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. ICR44 significantly blocks homotypic PNM aggregation on response to FMLP (3,4).Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Integrin alpha M (ITGAM) 抗体研究领域 Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
使用细节
应用备注 Flow Cytometry (use 10 μL of 1:10 - 1:100 diluted antibody to label 10^6 cells or 100 μL whole blood). Immunoprecipitation Immunohistochemistry on frozen sections (1:100 - 1:1000) Recommended positive control tissue: Human tonsil. Not suitable for paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
浓度 1.0 mg/mL
缓冲液 PBS, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the reconstituted antibody at 2-8 °C for one month (add 0.09% Sodium Azide) or at -20 °C for longer.
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