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- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
Vascular Cell Adhesion Molecule 1 (VCAM1) 抗体
- 规格:
0.1ml/0.2ml
Vascular Cell Adhesion Molecule 1 (VCAM1) 抗体
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibody
抗原
Vascular Cell Adhesion Molecule 1 (VCAM1)
别名 fi76d06, wu:fi76d06, zgc:158875, VCAM1, CD106, INCAM-100, Vcam-1, VCAM1B
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (M-K-2)
标记
其他选择 非结合性
应用范围
其他选择 流式细胞分析技术(FACS), 免疫组织化学(IHC), 免疫沉淀(IP)
Pubmed 2个引用可参考
规格 0.5 mg
发货至 中国 (更改)
产品细节Vascular Cell Adhesion Molecule 1 (VCAM1) 抗体
克隆位点 M-K-2
亚型 IgG1
产品特性 The antibody detects: CD106/VCAM-1 (approx. Mr 100 kDa)
The M/K-2 monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of approx. 100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
内毒素水平 Low Endotoxin
目标详细情况 Vascular Cell Adhesion Molecule 1 (VCAM1) 抗体
别名 CD106/VCAM-1
背景 VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein.
使用细节 Vascular Cell Adhesion Molecule 1 (VCAM1) 抗体
应用备注 Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
样本量 1 mL
限制 仅限研究用
贮存及处理 Vascular Cell Adhesion Molecule 1 (VCAM1) 抗体
缓冲液 Product is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
储存液 Azide free
注意事项 Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件 -20 °C
储存方法 Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
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文献和实验神经细胞粘着分子(neural cell adhesion molecule,N-CAM)
N-CAM是膜糖蛋白,至少以三种形式存在,但都是由同一基因编码。其中两种是跨膜蛋白,第三种共价结合在细胞质膜的外表面。无论是何种存在方式,N-CAM分子都有一部分伸出到细胞外表面。三种N-CAM在细胞外的结构都一样,都含有与细胞粘着相关的结合位点。 将分离纯化的N-CAM加入到人工磷脂脂质体中, 这种人工脂质体能够相互粘着,但是加入了相应的抗体就不会发生粘着, 不加N-CAM的人工脂质体也不会发生粘着。由此可以推测:细胞粘着是由细胞质膜中的蛋白分子介导的,N
Cell adhesion molecules (CAMs) such as intercellular adhesion molecule (ICAM-1), vascular adhesion molecule (VCAM-1), and lymphocyte function-associated antigen (LFA-1) facilitate interaction among cells of various lineages
【资源】[共享]Adhesion Protein Protocols——(很有用的)
Notes References 2— Epitope Mapping 3— Sequencing of Antibodies 4— Purification through Affinity Chromatography and Microsequencing 5— Functional Analysis of Cell Adhesion Molecules 6— Application of Polymerase Chain Reaction
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