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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
Ribosomal Protein L8 (RPL8) (C-Term) 抗体
- 规格:
0.1ml/0.2ml
Ribosomal Protein L8 (RPL8) (C-Term) 抗体
anti-Ribosomal Protein L8 (RPL8) (C-Term) antibody anti-Ribosomal Protein L8 (RPL8) (C-Term) antibody (2)
抗原
Ribosomal Protein L8 (RPL8)
别名 CG1263, Dmel\\CG1263, L8, M(3)62F, M(3)LS2, Rp L8, anon-EST:Posey5, rpL8, zgc:73105, zgc:77641
抗原表位
其他选择 C-Term
适用
其他选择 小鼠, 大鼠, 犬, 非洲爪蟾, 人, 斑马鱼
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 免疫组织化学(IHC), Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品细节 Ribosomal Protein L8 (RPL8) (C-Term) 抗体
免疫原 Synthetic peptide directed towards the C terminal of human RPL8
序列 KKVISSANRA VVGVVAGGGR IDKPILKAGR AYHKYKAKRN CWPRVRGVAM
预测反应 Chicken : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Zebrafish : 100 %, Bovine : 90 %
产品特性 This is a rabbit polyclonal antibody against RPL8. It was validated on Western Blot and immunohistochemistry.
纯化方法 Protein A purified
目标详细情况 Ribosomal Protein L8 (RPL8) (C-Term) 抗体
别名 RPL8
背景 Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL8 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
分子量 28 kDa
基因ID 6132
NCBI登录号 NP_000964, NM_000973
UniProt P62917
研究领域 Chromatin and Nuclear Signaling, DNA/RNA
使用细节 Ribosomal Protein L8 (RPL8) (C-Term) 抗体
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 257 AA
限制 仅限研究用
贮存及处理 Ribosomal Protein L8 (RPL8) (C-Term) 抗体
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
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文献和实验的亚型。市场上还有一种重组的Protein A和G的重组蛋白偶联的填料,即Protein A/G,它综合了二者的优点,使纯化过程更加方便。 另一个问题是IgM亚型的单抗并不是总是可以用Protein A来纯化的,一个比较好的办法是采用另一种亲和填料来纯化:Protein L resin,其原理和Protein A和G不太一样,Protein L可以特异性地结合抗体的Kappa链。在小鼠体内,至少95%的抗体是κ型的(另5%是λ型的),所以绝大部份单抗都可以用Protein L亲和填料纯化,其中就包括
members, including fibrillarin and nucleolin, involved in ribosomal biogenesis, histones, fibroblast growth factor, interleukin enhancer-binding factor 3 the SRC kinase adapter protein Sam68, STAT1, and EWS. Analysis of protein arginine methylation
Prediction of Protein‐Protein Interaction Networks
interacting protein families are first generated along with the 16S ribosomal RNA sequence alignments for the same taxa. (B ) Distance matrices are generated from the alignments (with tree‐of‐life distances subtracted from the distance matrices in the case
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