羧肽酶B1(CPB1)重组蛋白

羧肽酶B1(CPB1)重组蛋白 Recombinant Carboxypeptidase B1, Tissue (CPB1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No. RPF320Ra01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 37.5kDa
Predicted isoelectric point 6.5
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
羧肽酶B1(CPB1)重组蛋白
Subcellular Location Secreted
Residues Trp120~Tyr415 (Accession # P19223) with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- W ETIEAWIQQV ATDNPDLVTQ SVIGTTFEGR NMYVLKIGKT RPNKPAIFID CGFHAREWIS PAFCQWFVRE AVRTYNQEIH MKQLLDELDF YVLPVVNIDG YVYTWTKDRM WRKTRSTMAG SSCLGVRPNR NFNAGWCEVG ASRSPCSETY CGPAPESEKE TKALADFIRN NLSTIKAYLT IHSYSQMMLY PYSYDYKLPE NYEELNALVK GAAKELATLH GTKYTYGPGA TTIYPAAGGS DDWSYDQGIK YSFTFELRDT GFFGFLLPES QIRQTCEETM LAVKYIANYV REHLY
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity 羧肽酶B1(CPB1)重组蛋白to make location and size approximation of proteins of interest quick and easy.
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