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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
Wilms Tumor 1 (WT1) (N-Term) 抗体
- 规格:
0.1ml/0.2ml
Wilms Tumor 1 (WT1) (N-Term) 抗体
anti-Wilms Tumor 1 (WT1) (N-Term) antibody
抗原
Wilms Tumor 1 (WT1)
别名 WT1, WT, XWT1a, wt-1, wt1, xWT1, gud, wagr, wt33, xwt1, wit-2, AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33, XWT1b, XeWT1, CWT1, WT-1, D630046I19Rik, Wt-1, si:ch211-160j6.3, zgc:152697
抗原表位
其他选择 N-Term
适用
其他选择 人
宿主
其他选择 山羊
克隆类型
多克隆
标记
其他选择 非结合性
实验类型 Peptide ELISA
应用范围
其他选择 酶联免疫吸附测定(ELISA), Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 24至29个工作日
产品细节 Wilms Tumor 1 (WT1) (N-Term) 抗体
免疫原 Peptide with sequence QDPASTCVPEPASQH, from the N Terminus of the protein sequence according to NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3.
特异性 This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
纯化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
目标详细情况 Wilms Tumor 1 (WT1) (N-Term) 抗体
别名 Wilms tumor 1 / WT1
背景 Synonyms: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33
基因ID 7490
NCBI登录号 NP_077743, NP_077742, NP_000369, NP_077744
使用细节Wilms Tumor 1 (WT1) (N-Term) 抗体
应用备注 ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 55 kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3 kDa according to NP_000369.3). Recommended concentration: 1-3 µg/mL.
限制 仅限研究用
贮存及处理 Wilms Tumor 1 (WT1) (N-Term) 抗体
状态 Liquid
缓冲液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Minimize freezing and thawing.
储存条件 -20 °C
储存方法 Aliquot and store at -20 °C.
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文献和实验Wilms Tumor Gene WT1 as a: Tumor Marker for Leukemic Blast Cells and Its Role in Leukemogenesis
Patients with acute leukemia have a total of approx 1012 leukemic cells at the time of diagnosis, and still have as many as 1010 leukemic cells in complete remission. The most important problem in the treatment of leukemia patients
Mutational Analysis of the Wilms' Tumor (WTI) Gene
Mutations of the Wilms’ tumor (WT1) gene have been shown to underlie a proportion of cases of Wilms’ tumor, an embryonal kidney cancer occurring mainly in childhood. The WTl gene comprtses ten exons spanning approx 50 kb of genomrc DNA
WT1(Wilms’ tumor gene,WT1)定位于11p13,是最早发现与Wilms’肿瘤发生、发展有关的基因。 WT1主要在胚胎发生过程中表达,对泌尿生殖系统的发育起重要作用,在成人,WT1仅在肾脏、卵巢、子宫内膜、睾丸、脾脏、乳腺及正常造血祖细胞微量表达 [1]。 研究发现几乎所有的白血病原始细胞(无论系别)都持续过表达WT1,与其在极少数生理性造血干细胞(CD34 + )中短暂低表达形成鲜明对比,定性和半定
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