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文献和实验Column Purification of Demethylated Sphingomyelin
Packing column: 1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform. 2) Place a small portion of glass wool at the base of the column. 3) Pour gel solution into column and use a stir bar to press
Large Scale Tubulin Preparation
Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described here is for a large scale prep (10 cow brains
Column Purification of Demethylated Sphingomyelin
Column Purification of Demethylated Sphingomyelin Contributor: Suprya Jayadev Date: Mar. 11, 1991 Packing column: 1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform. 2) Place a small
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