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- Bio-Rad Laboratories
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- 2025年07月15日
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文献和实验Silver Stain is about 10 to 100 times more sensitive than Coomasie Blue Staining; at least 1ng protein using this protocol. Estimation of protein amouts is not advisable since the staining intensity depends of the developing time and vary
for microbial contamination before each use if not making fresh each time. 2. Solution A: Dissolve 100 mg silver acetate (Fluka Chemical Co., catalog # 85140) in 50 ml distilled water and keep under cover. To speed dissolving the fluffy powder, stir
.7)Rinse with water for 1 min.2X.0.1% Silver Nitrate and 0.08% Formalin (37%): For 1 gel,add 150 mg silver nitrate and 120μl 37% formalin to 150ml water.Developing: Must make fresh solution8)Incubate with 150ml 2% sodium carbonate with 0.04% formalin (37
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