- ￥69
- merck millipore
- TMS-004-C
- 2025年07月11日
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文献和实验Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
Sodium Acetate Precipitation of Small Nucleic Acids
sodium acetate. 3. Add 4 volumes of 100% ethanol; i.e. 1 ml 100% ethanol for 230 μl Lower Running Buffer plus 23 μl 3 M sodium acetate. 4. Mix thoroughly and incubate at –20° C overnight (16 hr). 5.
Phosphate (Sodium) buffer Chart
Phosphate (Sodium) buffer Chart Stock solution A 2 M monobasic sodium phosphate, monohydrate (276g/L) Stock solution B 2 M dibasic sodium phosphate (284 g/L). Mixing an appropriate volume (ml ) of A and B
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