重组人白介素-15（rHuIL-15）

Recombinant Human Interleukin 15

品名	rHuIL-15
规格	10μg、100μg
描述	重组人白介素-15蛋白是在大肠杆菌中表达的，是一条单链、非糖基化的多肽链，包含有114个氨基酸，4个半胱氨酸残基，形成2对链内二硫键。
来源	大肠杆菌。
分子量	理论分子量12.8kDa。
纯度	≥ 97%。
内毒素含量	﹤1EU/µg，鲎试剂法测定。
生物活性	用CTLL-2细胞株增殖的方法进行测定，比活性> 5 x 108IU/mg。
配方	本品为无菌冻干粉，缓冲体系为PBS, pH7.4，0.1%HSA。
复溶	建议开盖前以10,000rpm离心30-60秒，或3,000rpm离心5分钟，再使用0.2ml无菌水复溶。
保存	冻干样品自生产之日起在-20℃到-70℃可以稳定保存36个月。 溶解以后，该细胞因子能在2-8℃的无菌条件下保存一个月，或在-20℃到-70℃的人工除霜的冰箱中保存三个月，而无明显活性损失。 避免反复冻融。

Interleukin 15 (IL-15) is a widely expressed cytokine that is structurally and functionally related to IL-2. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly. IL-15 binds with high affinity to IL-15Rα. It binds with lower affinity to a complex of IL2Rβ and the common gamma chain (γc) which are also subunits of the IL-2 receptor complex. IL-15 associates with IL15Rα in the endoplasmic reticulum, and this complex is expressed on the cell surface. The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15Rα are coordinately expressed on the surface of one cell and interact with complexes of IL2Rβ/γc on adjacent cells. This enables cells to respond to IL-15 even if they do not express IL-15Rα. Soluble IL-15 binding forms of IL-15Rα can be generated by proteolytic shedding or alternate splicing. These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function. Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2 like effects in lymphocyte development and homeostasis. It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells. IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells. Ligation of membrane associated IL-15/IL-15Rα complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL15/IL15Rα expressing cells.
仅供实验室使用。