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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
dCTP,PCR Grade Di-Na solution
- 供应商：
上海汇谱仪器有限公司
- CAS号：
459130-BR
- 规格：
1,250 µl (125 µmol)
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文献和实验Carbonate Solution For Western Blot
0.84g 3mM Na2CO3 0.477g pH ~9.9 0.318g in 20% MeOH 300mL 200mL 1. Blot
b-galactosidase Solution Assay
of 0.25 M Tris HCl pH 8.0. • Freeze thaw the cells 3 times by alternating between dry ice and 37°. • Spin for 15 minutes at 4° and transfer the supernatant to a new tube and hold on ice. • Mix the following reaction solution per sample: 264
Troubleshooting for PCR and multiplex PCR
some/all of the above 3. Reaction was working before, but now I can't get any product. Make sure all PCR ingredients are taken in the reaction (buffer, template, Taq, etc) Change the dNTP solution (very sensitive to cycles of thawing and freezing, especially in multiplex PCR)
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