- ¥519
- promega
- 进口
- Q6311
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
30µl
| Q6311 | Ampicillin Repair Oligonucleotide | 30µl | 519 | |
| Q6321 | Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent) | 500µl | 2,531 | |
| Q6700 | T7 EEV Promoter Primer | 2µg | 618 | |
| R1851 | 10X Flexi® Enzyme Blend (Sgf I and Pme I) | 25µl | 958 | |
| R1852 | 10X Flexi® Enzyme Blend (Sgf I and Pme I) | 100µl | 3,233 | |
| R1901 | Carboxy Flexi® Enzyme Blend(SgfI & EcoICRI) | 50µl | 1,144 | |
| R4014 | EcoR I (HC) | 25,000u | 621 | 311 |
| R4017 | EcoR I (HC) | 50,000u | 1,025 | 513 |
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文献和实验In vitro mutagenesis using Altered Sites
synthesis 1.Add to an ependorf: 100ng ssDNA 1μl 2.2ng/µl phosphorylated ampicillin repair oligo 1μl 10ng/µl phosphorylated mutagenic oligo 2μl 10x annealing buffer dH2O to 20µl 10x annealing buffer 200mM Tris-Cl pH 7.5 100mM MgCl2 500mM NaCl
. The second stepinvolves DNA repair process activated by the targeted cleavage,carried out normally by the endogenous DNA damage repair machinery within the cells. In this step, two major pathways couldbe employed, achieving different types of genome modifi
five DNA aliquots (approximately 50 ug) are sonicated according to those pre-determined conditions. After sonication, the five tubes are placed in an ice-water bath until fragment end-repair and size selection, discussed below. Protocol 1. Prepare
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