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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- 规格：
2 x 250 µL
Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Features of PageRuler Prestained Protein Ladder:
• Size range—10 proteins spanning 10 to 180 kDa
• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
• Sharp bands—color-coded bands of similar intensity for easy visualization
• Quality tested—each lot evaluated by SDS-PAGE and Western blotting
• Two reference bands—orange at 70kDa and green at 10kDa
• Membrane-compatible—colored bands transfer to membranes for Western blotting
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.
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文献和实验(2 μl) RNA ladder with 5 μg anchored Oligo(dT)20 primer at 70°C for 5 minutes. Keep on ice. 2) Combine the RNA-oligo dT complex from Step 1 with 1X 1st strand cDNA synthesis buffer supplemented with 10 mM DTT, 500 μM dNTP, 2 U RNaseOUT™
into the wells of the SDS-PAGE gel. Load an appropriate amount of Trident Blue Prestained Protein Ladder or Trident Prestained Protein Ladder to one or more additional lanes.2. Run the gel in 1X Trident Running Buffer for 1-2 hours at 50-100 V.We recommend
Literature Cited Abstract Native gels enable the analysis of protein complexes on a proteome
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