- 询价
- 沪震生物
- 进口/国产
- hz452Hu01
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Myosin Light Chain 9, Regulatory (MYL9
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPD452Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 19.9kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
肌球蛋白轻链9(MYL9)重组蛋白
Subcellular Location n/a
Residues Arg5~Leu163 (Accession # P24844) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-RAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RIL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity 肌球蛋白轻链9(MYL9)重组蛋白 to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验以达到纯化抗体IgG的目的。一般管中收集的IgG抗体用蛋白胶（SDS-PAGE电泳）进行检测，并判断IgG的纯度和浓度。图4 精达公司重组蛋白A纯化柱产品 图5为广州精达公司蛋白A层析柱纯化后所制备的蛋白胶（SDS-PAGE电泳胶）。图5中，经精达公司层析柱洗脱后，从第1管至第8管均有抗体的重链和轻链的带型。将上述有蛋白带型的管进行合并后测量抗体的浓度，广州精达公司蛋白A平均抗体IgG的载率为52mg/ml。 上述IgG载率在诊断试剂及成本中有着十分重要的意义，众所周知，目前
填料 根据亲和层析的几种应用方向，亲和层析的填料主要分为标签蛋白纯化填料、抗体及抗体片段纯化填料、族特异性亲和填料以及预活化填料四种。 1. 标签蛋白纯化填料 为了简化纯化流程以及方便后期的检测，在进行重组蛋白表达时往往会加入标签。常用的标签包括His标签、GST标签、Strep标签及MBP标签等 （表8)。His标签由于分子量很小，几乎不会影响靶蛋白结构和功能而被广泛使用。GST和MBP标签常用于促进蛋白的可溶表达，尤其对于难表达的真核细胞蛋白和病毒蛋白有很好的促溶表达能力。Strep
L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白，兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G，也可根据结合力表格进行选择，详见资源信息部分 3、基质—琼脂糖还是磁珠？ 琼脂糖 磁珠 优点 简单易用，无需辅助设备 操作简单 孔径大，直径大（50-150
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