- ¥2026
- promega
- 进口
- L2005
- 2026年01月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
1ml (20 × 50µl)
| L2005 | Single-use JM109 Competent Cells, >108cfu/µg | 1ml (20 × 50µl) | 2,026 | |
| L2011 | HB101 Competent Cells, >108cfu/µg | 1ml | 990 | |
| L2015 | Single-use HB101 Competent Cells | 1ml (20 × 50µl) | 1,771 | |
| L2080 | TNT® SP6 Quick Coupled Transcription/Translation System | 40 reactions | 4,819 | |
| L2081 | TNT® SP6 Quick Coupled Transcription/Translation System, Trial Size | 5 reactions | 1,320 | |
| L3002 | Single Step (KRX) Competent Cells | 20 × 50µl | 2,501 | |
| L3260 | TNT® SP6 High-Yield Protein Expression System | 40 reactions | 5,446 | |
| L3261 | TNT® SP6 High-Yield Protein Expression System | 10 reactions | 1,694 | |
| L4130 | TNT® SP6 Coupled Wheat Germ Extract System | 40 reactions | 4,433 | |
| L4140 | TNT® T7 Coupled Wheat Germ Extract System | 40 reactions | 4,433 |
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文献和实验TOP10 chemically competent cells
should yield around 100 - 400 colonies Transformation efficiency is (dilution factor=15) x colony count x 105 /µgDNA We expect that the transformation efficiency should be between 5x108 and 5x109 cfu/µgDNA 5x Ligation Adjustment
Preparation of Competent Cells (from PMB)
the cells again (5 K, 5 min, 4 ℃). 4.Decant and resuspend in 1 ml PMB #B. Then add 9 ml more PMB #B. 5.Let sit on ice for 30 min, spin as above and resuspend in 2 ml PMB #B, add 0.2 ml glycerol and 30 µl DMSO. Use fresh or freeze in dry ice/EtOH. PMB
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
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