- ¥10356
- promega
- 进口
- L1210
- 2026年01月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
1 each
| L1210 | TnT® T7 Quick Starter Bundle, Chemiluminescent | 1 each | 10,356 | ||
| L1215 | TnT® T7 Quick Starter Bundle, Colorimetric | 1 each | 9,102 | ||
| L2001 | JM109 Competent Cells, >108cfu/µg | 1ml | 1,151 | ||
| L2005 | Single-use JM109 Competent Cells, >108cfu/µg | 1ml (20 × 50µl) | 2,026 | ||
| L2011 | HB101 Competent Cells, >108cfu/µg | 1ml | 990 | ||
| L2015 | Single-use HB101 Competent Cells | 1ml (20 × 50µl) | 1,771 | ||
| L2080 | TNT® SP6 Quick Coupled Transcription/Translation System | 40 reactions | 4,819 | ||
| L2081 | TNT® SP6 Quick Coupled Transcription/Translation System, Trial Size | 5 reactions | 1,320 | ||
| L3002 | Single Step (KRX) Competent Cells | 20 × 50µl | 2,501 | ||
| L3260 | TNT® SP6 High-Yield Protein Expression System | 40 reactions | 5,446 | ||
| L3261 | TNT® SP6 High-Yield Protein Expression System | 10 reactions | 1,694 | ||
| L4130 | TNT® SP6 Coupled Wheat Germ Extract System | 40 reactions | 4,433 | ||
| L4140 | TNT® T7 Coupled Wheat Germ Extract System | 40 reactions | 4,433 | ||
| L4151 | Rabbit Reticulocyte Lysate, Untreated | 1ml | 1,149 | ||
| L4330 | Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System | 24 reactions | 1,793 |
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文献和实验The ribonuclease protection assay (RPA)
P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix
: RXR without DNA, your wash buffer must be 1x PBS supplemented with 500mm NaCl. Material and solutions: IPAB-gelatin: HEPES 20mM pH7.8 KCl 150mM Gelatin 0.1% TritonX100 0.1% NP40 0.1% MgCl2 5mM DTT 2mM Wash buffer: PBS 1X DTT 2mM TnT Quick T7
RNase Protection Assay（RPA)操作步骤
4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate
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