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- promega
- 进口
- L1171
- 2026年07月22日
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- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
5 reactions
| L1171 | TNT® T7 Quick Coupled Transcription/Translation System, Trial Size | 5 reactions | 1,307 |
| L1191 | BL21(DE3)pLysS Competent Cells, >106cfu/µg | 1ml | 1,410 |
| L1195 | Single-use BL21(DE3)pLysS Competent Cells | 1ml (20 × 50µl) | 3,416 |
| L1210 | TnT® T7 Quick Starter Bundle, Chemiluminescent | 1 each | 10,356 |
| L1215 | TnT® T7 Quick Starter Bundle, Colorimetric | 1 each | 9,102 |
| L2001 | JM109 Competent Cells, >108cfu/µg | 1ml | 1,151 |
| L2005 | Single-use JM109 Competent Cells, >108cfu/µg | 1ml (20 × 50µl) | 2,026 |
| L2011 | HB101 Competent Cells, >108cfu/µg | 1ml | 990 |
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文献和实验The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
at the level of transcription, including Northern blot hybridization, RNase protection and reverse transcription coupled with polymerase chain reaction amplification (RT-PCR). RT-PCR is a sensitive and versatile method which can be used to determine
transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32P]UTP 1 µl T7 RNA
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