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- promega
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- L1170
- 2026年07月22日
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- 保存条件：
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- 供应商：
上海善然生物科技有限公司
- 规格：
40 reactions
| L1170 | TNT® T7 Quick Coupled Transcription/Translation System | 40 reactions | 4,819 |
| L1171 | TNT® T7 Quick Coupled Transcription/Translation System, Trial Size | 5 reactions | 1,307 |
| L1191 | BL21(DE3)pLysS Competent Cells, >106cfu/µg | 1ml | 1,410 |
| L1195 | Single-use BL21(DE3)pLysS Competent Cells | 1ml (20 × 50µl) | 3,416 |
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文献和实验Assembling Linear DNA Templates for In Vitro Transcription and Translation
Cell-free expression systems provide straightforward access from genes to the corresponding proteins, involving fewer handling steps than in vivo procedures. A quick procedure to assemble a gene of interest into a linear DNA template
could be used. Preclearing the immunoprecipitation with protein A/G sepharose is also a possibility. Prepare proteins separately by in vitro transcription and translation according to the standard Promega TNT reticulocyte protocol. Adjust total reaction
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
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