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- 钰博生物
- 进口/国产
- yb135483
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
Monocyte/Granulocyte 抗体 (PE)
- 规格：
0.1ml/0.2ml
Monocyte/Granulocyte
适用
其他选择 猪
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (74-22-15)
标记
其他选择 PE
应用范围
其他选择 流式细胞分析技术（FACS）, 免疫组织化学（IHC）
Pubmed 找到1个引用
规格 0.1 mg
发货至 中国 (更改)
发货时间 23至28个工作日
产品编号 yb135483
产品细节 Monocyte/Granulocyte 抗体 (PE)
克隆位点 74-22-15
亚型 IgG1
产品特性 The antibody detects: Porcine SWC3a
MAb 74-22-15 reacts with > 90 % of pig monocytes and granulocytes and < 5 % of peripheral blood lymphocytes. This clone was clustered as anti-SWC3a at the First International Swine CD Workshop.
纯化方法 Rabbit Anti-Cofilin (Phospho-Ser3) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
使用细节 Monocyte/Granulocyte 抗体 (PE)
应用备注 Working Dilution:
FACS: 02 µg/10^6 cells
Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
样本量 1 mL
限制 仅限研究用
贮存及处理 Monocyte/Granulocyte 抗体 (PE)
缓冲液 Product is supplied as 0.1 mg in 1.0 mL of PBS / NaN3 and a stabilizing agent.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件 4 °C
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文献和实验抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
PriCells: 藻红蛋白标记抗体的方法﹠PE-标记蛋白A方法
藻红蛋白标记抗体的方法：1. 巯基化藻红蛋白（PE）的制备；（1）将600μl浓度为15.5mg/ml的盐酸巯醇亚胺加到1.2ml浓度为3.6mg/ml的PE中；（2）将以上混合液和1.2ml pH6.8的PB混合，而后装入透析袋置入50mmol/L pH6.8的PB中于4℃透析，过夜；（3）再换用pH7.5 PB透析6h。每个PE分子中可结合8个巯基；2. 巯基PE-IgG的制备；（1）将30μl浓度为1.1mg/ml的异双功能试剂SPDP的乙醇溶液，加入到700μl
: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation
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