Heat Shock 70kDa Protein 8 (HS

Heat Shock 70kDa Protein 8 (HSPA8) 抗体抗原
Heat Shock 70kDa Protein 8 (HSPA8)
别名 hsc54, hsc70, hsc71, hsp71, hsp73, hspa10, lap1, nip71, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, NIP71, Hsc70, 2410008N15Rik, Hsc71, Hsc73, Hsp73, Hspa10, wu:fb01g06, wu:fi48b06
适用
其他选择 Beluga, 牛, 犬, Caenorhabditis elegans (C. elegans), 小鸡, Fruit Fly (Drosophila melanogaster), 鱼, 豚鼠, Hamster, 人, 猴, 小鼠, 植物, 猪, 兔, 大鼠, 绵羊, 非洲爪蟾
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (N27F3-4)
标记
其他选择 非结合性
应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM), 流式细胞分析技术（FACS）, 免疫萤光（IF）, 免疫沉淀（IP）, Western Blotting (WB)
Pubmed 7个引用可参考
规格 50 μg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb263940
产品细节 Heat Shock 70kDa Protein 8 (HSPA8) 抗体
免疫原 Recombinant hsp70/hsc70 (1)
克隆位点 N27F3-4
亚型 IgG1
特异性 Detects 72 and 73 kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.
产品特性 Synonyms: HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8
纯化方法 Affinity Chromatography on Protein G.
目标详细情况Heat Shock 70kDa Protein 8 (HSPA8) 抗体
别名 HSPA8 / HSC70
背景 Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.Synonyms: HSP70.1, HSP70.2, HSP73, HSPA10, HSPA1A, HSPA1B, HSPA8, Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein
基因ID 3312
UniProt P11142
使用细节 Heat Shock 70kDa Protein 8 (HSPA8) 抗体
应用备注 Western blot (7,8,9): 1 μg/mL was sufficient for detection of HSP/HSC70 in 20 μg of HeLalysate. Immunoprecipitation (7). Immunohistochemistry (8). Flow Cytometry. Electron Microscopy (8).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 Heat Shock 70kDa Protein 8 (HSPA8) 抗体
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Shelf life: one year from despatch.
有效期 12 months