Macrophage F4/80 Gen 抗体

Macrophage F4/80 Gen 抗体抗原
Macrophage F4/80 Gen
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (Cl:A3-1)
标记
其他选择 非结合性
应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoelectron Microscopy (IEM), 流式细胞分析技术（FACS）, 免疫萤光（IF）, 免疫沉淀（IP）, Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Radioimmunoassay (RIA), Western Blotting (WB), 免疫组织化学石蜡技术（IHC-P）
Pubmed 13个引用可参考
规格 0.5 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb111846
产品细节 Macrophage F4/80 Gen 抗体
免疫原 Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
克隆位点 Cl:A3-1
亚型 IgG2b
特异性 This antibody recognises the F4/80 antigen, a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EMR1. Clone CI:A31 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5). We recommend the use of BM4008LE for this purpose.
交叉反应 （详细） Species reactivity (tested):Mouse.
纯化方法 Affinity Chromatography on Protein G
目标详细情况 Macrophage F4/80 Gen 抗体
别名 Macrophage F4/80 Antigen
背景 F4/80 antigen is a 160 kD glycoprotein expressed by most murine macrophages. Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymphnode. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
基因ID 13733
NCBI登录号 NP_034260
UniProt Q61549
使用细节 Macrophage F4/80 Gen 抗体
应用备注 RIA. Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded and Resin Sections. Thisproduct requires pre-treatment of paraffin sections (Proteinase K is recommended fortissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixedfor more than 24 hours).
限制 仅限研究用
贮存及处理 Macrophage F4/80 Gen 抗体
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.