MHC Class I Qa.m2 抗体

MHC Class I Qa.m2 抗体抗原
MHC Class I Qa.m2
适用
小鼠
宿主
小鼠
克隆类型 (克隆位点)
单克隆 (141-16-6)
应用范围
Cytotoxicity Test (CyTox)
Pubmed 6个引用可参考
规格 0.5 mL
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114300
产品细节 MHC Class I Qa.m2 抗体
免疫原 Recipient: C58/J Donor: BALB/c-H-2b Fusion Partner: P3-NSI/1-Ag4(NS-1)
克隆位点 141-16-6
亚型 IgG2b
特异性 This monoclonal antibody detects the Qa.m2 antigenic determinant on the Qa-2 Molecule. Thus, although the role of the Qa-2 antigen is unclear, antibodies to this antigen may be useful tools for separating functionally distinct subpopulations of lymphocytes and resolving pathways of differentiation. It may prove to be a useful marker in the study of B lymphocyte differentiation as well as differentiation of certain T cell functional populations.
纯化方法 Ascites
目标详细情况 MHC Class I Qa.m2 抗体
背景 The Qa region, so named because of the closely linked Qa 1-9 loci, is situated on murine chromosome 17 between the H-2D and T1a loci. These Qa loci code for cell surface alloantigens expressed on some lymphoid and myeloid cells. Their expression appears to be controlled by a gene or genes closely linked to the H-2D locus and they have been identified as being structurally related to the murine H-2 antigens. Although no functional role for Qa antigens has been determined, the restricted and differential distribution of Qa determinants on hemopoietic and lymphoid cells raises the possibility that they may not be simply differentiation markers but may play a role in hematopoetic differentiation. The Qa-2 Molecule has been identified as a two-chain structure composed of a 39,000 d heavy chain and a 12,000 d light chain which is B2 microglobulin. (The heavy chain is readily distinguished from that of H-2.) The Qa.m2 antigen has a wide representation on hematopoeitic cells. It is however, found predominantly on peripheral T cells (cytotoxic and helper T cells) but was present on only 10 % of thymus cells. Subpopulations of B cells (approximately 25 % of all B cells) and bone marrow cells (15-20 %) are also reactive. Qa.m2 was also detected on megakaryocyte colony-forming cells (MEG-CFC) but not on granulocyte- macrophage, erythroid or eosinophil colonies. In addition, the Qa.m2 is a marker of T cell differentiation, as it is selectively expressed on functional T cells. The control of Qa-2 antigen expression and of other Qa antigens by the H-2 region may reflect a control mechanism of functional T cell differentiation and ontogeny in developing T lymphocytes.
基因ID 110557
NCBI登录号 NP_997531
UniProt P79568
研究领域 Major Histocompatibility Complex (MHC), Immunology
使用细节 MHC Class I Qa.m2 抗体
应用备注 Cytotoxicity Analysis. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF Qa. m2 POSITIVELYMPHOCYTES1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-M density cell separation medium . After washing adjust thecell concentration to 1 x 10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration 1: 20 and mix. Alternately, pellet the cells andresuspend in antibody diluted 1: 20 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Low-Tox®-M Rabbit Complement3, diluted to theappropriate concentration in Cytotoxicity Medium. (Recommended concentration included
限制 仅限研究用
贮存及处理 MHC Class I Qa.m2 抗体
状态 Liquid
溶解方式 Restore with 0.5 mL of distilled water.
注意事项 Use of Sodium Azide as a preservative will substantially inhibe the activity of Horseradish peroxidase (HRP).
注意事项 Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
储存条件 -20 °C
储存方法 Prior to reconstitution store the antibody at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or at -20 °C for longer.