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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 目录编号:
252275
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
见详细说明信息
- 靶点:
MHC Class I (HI21)
- 抗体英文名:
MHC Class I (HI21) Antibody
- 抗体名:
MHC Class I (HI21) Antibody
- 规格:
2 ml
MHC Class I (HI21) Antibody产品详细介绍:
| 产品名称: | MHC Class I (HI21) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252275 |
| 规格: | 2 ml |
| 产品描述: | MHC Class I (HI21) antibody recognizes class I major histocompatibility complex (or human leukocyte antigen) monomorphic antigen which is expressed widely on human nucleated cells such as lymphocytes, monocytes/ macrophages, dendritic cells and other hematopoietic and non-hematopoietic nucleated cells, and platelets. The expression of HLA-I antigen varies with cell types and decreases with cell maturation, e.g. low expression on mature granulocytes and almost null on mature erythrocytes. HLA-I antigen is also expressed on most cells of human leukemic cell lines, but not or low in Daudi and K562 cell lines and nerve cells. In addition to membrane HLA-I antigen, a soluble form of HLA-I can be detected in serum. |
| 克隆类型: | Mouse Monoclonal Antibody, FITC-conjugated Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | |
| 适用物种: | H |
| 应用范围: | FC |
| 应用说明: | FC with whole blood: 100 μl blood + 20 μl antibody; FC with cells: 10E6 cells + 20 μl antibody. FC: 1:200-1:1,000 |
| 别名: | HLA-I; HLA class I histocompatibility antigen; MHC class I antigen |
| 形态: | Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: | Chen Z., et al. 1987. Chinese Science Bulletin. 24: 1902; McMichael JA., et al. eds. 1986. Leucocyte Typing III: White Cell Differentiation Antigens. P59, 70. Garland Publishing, Inc., New York. |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验抗原,I region associated antigen): Ir基因编码产物二. 人类HLA复合体受体: (1)kidney移植,排斥 (2)接受多次输血血液中含有与供体白细胞反应的抗体靶分子:MHC(有核细胞表面)人白细胞抗原Human leucocyte antigen, HLA (一). 人类HLA复合体的定位和结构定位:第6号染色体短臂(6p21,3)结构:十分复杂(多基因性polygeny和多态性polymorphism)分成三类基因区: (224个基因座locus, 128个功能
板,利用 ELISA 方法测定抗体亲和力,可达到高通量检测效果。Anna Sergeeva 等(2011)在研究人类急性髓细胞样白血病的 TCR 样抗体工作中,用 HLA-A2-PR1 多肽复合物免疫小鼠,分离小鼠脾脏细胞。培养单克隆细胞,制备抗体。将 MHC-PR1 多肽复合物包被于酶联板上,进行同量化 ELISA 筛选。在 2850 个克隆中,筛选到一个(8F4)具备亲和力的抗体。推荐阅读:1. TCR Mimic Monoclonal Antibody Targets a Specific
免疫细胞膜分子(一):主要组织相容性抗原-- MHC基因结构
(histocompatibility antigen)或移植抗原(transplantation antigen)。机体内与排斥反应有关的抗原系统多达20种以上,其中能引起强而迅速排斥反应者称为主要组织相容性抗原,其编码基因是一组紧密连锁的基因群,称为主要组织相容性复合体(major histocompatibility complex,MHC)。现已证明,控制机体免疫应答能力与调节功能的基因(immune uesponse gene,Ir gene )也存在于MHC内。因此,MHC不仅与移植排斥反应有关,也广泛参与免疫
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