Left-Right Determination Facto

Left-Right Determination Factor 2 (LEFTY2) 抗体抗原
Left-Right Determination Factor 2 (LEFTY2)
别名 atv2, cb720, fe38b03, wu:fe38b03, LEFTY1, AI450052, Ebaf, Leftb, Stra3, Tgfb4, lefty, lefty-1, EBAF, LEFTA, LEFTYA, TGFB4, 6030463A22Rik, AV214969, Lefta, Ebaf2
适用
其他选择 人, 小鼠, 大鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (7C5G1H6H10)
标记
其他选择 非结合性
应用范围
其他选择 酵素免疫分析法（EIA）, Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), 免疫组织化学石蜡技术（IHC-P）
Pubmed 4个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb116931
产品细节 Left-Right Determination Factor 2 (LEFTY2) 抗体
免疫原 A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
克隆位点 7C5G1H6H10
亚型 IgG2a
特异性 This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
纯化方法 Protein A chromatography.
目标详细情况 Left-Right Determination Factor 2 (LEFTY2) 抗体
别名 LEFTY2
背景 During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.Synonyms: EBAF, Endometrial bleeding-associated factor, LEFTA, LEFTY2, LEFTYA, Left-right determination factor 2, Left-right determination factor A, Protein lefty-2, Protein lefty-A, TGF-beta-4, TGFB4, Transforming growth factor beta-4
基因ID 7044
NCBI登录号 NP_003231
UniProt O00292
研究领域 Cancer, Growth Factors
使用细节 Left-Right Determination Factor 2 (LEFTY2) 抗体
应用备注 This antibody is suitable for ELISA (1/5,000) and Western blotting (1/2,000). The antibodymay be used for other applications, such as RIA, Immunohistochemistry orImmunoprecipitation, but specific reaction conditions have not been developed.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理Left-Right Determination Factor 2 (LEFTY2) 抗体
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 4 °C/-20 °C
储存方法 Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.