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- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
CD72 Molecule (CD72) 抗体 (FITC)
- 规格：
0.1ml/0.2ml
CD72 Molecule (CD72) 抗体 (FITC)抗原
CD72 Molecule (CD72)
别名 CD72c, Ly-19, Ly-32, Ly-m19, Lyb-2, CD72b, LYB2
适用
其他选择 小鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (CT-72-1)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 4个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114238
产品细节 CD72 Molecule (CD72) 抗体 (FITC)
克隆位点 CT-72-1
亚型 IgG2a
特异性 This antibody reacts with the CD72 alloantigen CD72.1, a B-cell surface protein that is encoded by the Cd72a allele. CD72.1 is expressed on cells of the B cell lineage, except plasma cells3. Mouse strains expressing CD72.1 include C57L/-, C58/-, DBA/1, DBA/2, and SWR/J. Tissue Distribution by Flow Cytometry Analysis: (Representative Dot Plot) Mouse Strain Tested: DBA/2 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.25 μg/10e6 cells Isotypic Control: FITC Mouse IgG2a.
目标详细情况 CD72 Molecule (CD72) 抗体 (FITC)
别名 CD72
背景 CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
基因ID 100504743
NCBI登录号 XP_003086403
UniProt P21855
研究领域 Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
使用细节 CD72 Molecule (CD72) 抗体 (FITC)
应用备注 Flow cytometry (For details please see protocols / specifity below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 25 µg of antibody per 1 x 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
限制 仅限研究用
贮存及处理CD72 Molecule (CD72) 抗体 (FITC)
浓度 0.1 mg/mL
缓冲液 PBS, 0.09 % sodium azide (NaN3) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD72 Molecule (CD72)
别名 CD72c, Ly-19, Ly-32, Ly-m19, Lyb-2, CD72b, LYB2
适用
其他选择 小鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (CT-72-1)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 4个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114238
产品细节 CD72 Molecule (CD72) 抗体 (FITC)
克隆位点 CT-72-1
亚型 IgG2a
特异性 This antibody reacts with the CD72 alloantigen CD72.1, a B-cell surface protein that is encoded by the Cd72a allele. CD72.1 is expressed on cells of the B cell lineage, except plasma cells3. Mouse strains expressing CD72.1 include C57L/-, C58/-, DBA/1, DBA/2, and SWR/J. Tissue Distribution by Flow Cytometry Analysis: (Representative Dot Plot) Mouse Strain Tested: DBA/2 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.25 μg/10e6 cells Isotypic Control: FITC Mouse IgG2a.
目标详细情况 CD72 Molecule (CD72) 抗体 (FITC)
别名 CD72
背景 CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.Synonyms: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
基因ID 100504743
NCBI登录号 XP_003086403
UniProt P21855
研究领域 Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
使用细节 CD72 Molecule (CD72) 抗体 (FITC)
应用备注 Flow cytometry (For details please see protocols / specifity below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 25 µg of antibody per 1 x 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
限制 仅限研究用
贮存及处理CD72 Molecule (CD72) 抗体 (FITC)
浓度 0.1 mg/mL
缓冲液 PBS, 0.09 % sodium azide (NaN3) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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文献和实验相关实验
CD72 分子 CD72 常用单克隆抗体或代号： S- HCL2,J3.109,BU- 40 主要表达细胞： B [B] 分子质量(kDa)和结构： gp43,39(C型凝集素) 功 能： 与CD5结合,B细胞活化、增殖 CD72 Cell surface protein expressed exclusively on B cells; may control B cell
CD5 分子 CD5 常用单克隆抗体或代号：T1，UCHT2，T101，Leul 主要表达细胞：T，Thy，BsubC T细胞 分子质量(kDa)和结构：gp67(清除剂受体) 功 能：与CD72结合，T细胞信号转导和增殖，CD5+ B细胞与自身免疫有关 CD5 Belongs to ancient scavenger receptor family
，用含有10%热灭活胎牛血清的RPMI1640培养基于5% CO2，37C条件下培养。抗CD20单克隆抗体HI47由我所免疫室提供。辣根过氧化酶标记羊抗人IgG，FITC标记的羊抗鼠IgG购于北京中山生物工程公司。2、抗CD20单克隆抗体轻、重链基因的克隆利用RT-PCR法，采用抗体通用的兼并性引物，从HI47杂交瘤细胞特异性地分别扩增抗体轻链、重链可变区基因（VL和VH）后，利用编码短肽（G4S）3的连接链DNA片段，经overlap PCR，将VH和VL基因连接构成ScFv基因（约750bp
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CD72 Molecule (CD72) 抗体 (FITC)
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