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100 x 50 µl rxns
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文献和实验实验步骤 1. Protocol for grinding plant seeds. 1) grind using a bead mill a. place one seed into each well of a 2 ml square well block. b. Pipette nuclease-free water
Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR
Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method
and synthesis of first-strand cDNA (ref. 60), allowing the construction of solid-phase cDNA libraries and solid-phase RT-PCR. 实验试剂 Lysis/Binding Buffer Washing Buffer A Washing Buffer B 10 mM Tris-HCl (Elution Buffer
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