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文献和实验【求助】topflash/foplash的luciferase opening reading fream针对的就是firefly萤光素酶吗？
orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. 这里的Luciferase没有标明是Firefly的啊，那我要不要再转一个PGL3载体呢？？？ 在线等答案，谢谢啦！ paroxysm 荧光素酶已经带上了，但是不是firefly种属，看说明书上说的。 但kit里的底物应该是针对这个荧光素酶的。
Improvements in the Application of Firefly Luciferase Assays
The firefly luciferase-based assay differs from most familiar enzyme-based determinations. Most enzyme assays are based either on the production of a product or the disappearance of a substrate. Usually the compound measured is stable
Application of the Firefly Luciferase Reporter Gene
) as a reporter gene proved to be both laborious and onerous. In contrast, the luciferase gene possesses intrinsic qualities that most other reporter genes lack greater sensitivity, ease of use, instantaneous quantification, “ environment friendliness
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