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文献和实验Basic Protein Sequence Analysis
for Understanding Results Predicting the Subcellular Localization of a Protein Using TargetP Basic Protocol 3: Predicting Key Functional Residues and Motifs using the Prosite Web Server Support Protocol 6: Guidelines
Basic Protein Chemistry Techniques
Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2 O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use, add 50 ml acetic acid to 10% final concentration. 4) Stain for 2 hours. Staining time
The COS-7 Cell In Vitro Paradigm to Study Myelin Proteolipid Protein 1 Gene Mutations
Despite many shortcomings, a reductionist approach using cell culture paradigms to define basic principles underlying disease processes has considerable merit. One example of the utility of this approach is the expression of mutant forms
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