- 询价
- 沪震生物
- 进口/国产
- hz310Hu01
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Adenine Phosphoribosyltransferase (APRT)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPC310Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 23.4kDa
Predicted isoelectric point 6.8
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Sub腺嘌呤磷酸核糖转移酶(APRT)重组蛋白cellular Location Cytoplasm
Residues Met1~Glu180 (Accession # P07741) with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
腺嘌呤磷酸核糖转移酶(APRT)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验佚名 大多数细胞更新其核酸(尤其是RNA)过程中，要分解核酸产生核苷和游离碱基。细胞利用游离碱基或核苷重新合成相应核苷酸的过程称为补救合成(saluage pathway)。与从头合成不同，补救合成过程较简单，消耗能量亦较少。由二种特异性不同的酶参与嘌呤核苷酸的补救合成。腺嘌呤磷酸核糖转移酶(Adenine phosphoribosyl transterase
acridine orange 全名（中） 吖啶橙 含义 名 称 APRT 全名（英） adenine phosphoribosyltran-sferase 全名（中） 腺嘌呤磷酸核糖转移酶 含义 名 称 APT 全名（英） aminopterin 全名（中） 氨基蝶呤 含义 名 称 ATCC 全名（英） American Tissue
heterochromatin|兼性异染色质 facultative parthenogenesis|兼（性）孤雌生殖 faint band|模糊条带 familial adenomatous polyposis|家族性多发性腺癌[FAP是一种抗癌基因] far infrared|远红外 farnesol|法尼醇，麝子油醇 farnesyl transferase|法尼基转移酶
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