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- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
10µl
| G6570 | HeLa Nuclear Extract | 10µl | 1,393 |
| G6591 | HaloTag® Control Vector | 20µg | 2,331 |
| G6601 | HaloTEV Protease | 1,000u | 1,254 |
| G6602 | HaloTEV Protease | 4,000u | 3,785 |
| G6790 | HaloTag® Mammalian Protein Purification System | 1 each | 6,316 |
| G6795 | HaloTag® Mammalian Protein Detection and Purification System | 1 each | 7,582 |
| G6799 | HaloTag® Mammalian Protein Detection and Purification System Sample Pack | 1 each | 3,355 |
| G6941 | 1kb DNA Step Ladder | 90µg | 893 |
| G6951 | 100bp DNA Step Ladder | 100µg | 1,417 |
| G6961 | 200bp DNA Step Ladder | 100µg | 1,123 |
| G7061 | rhSkin ß Tryptase | 100µg | 4,772 |
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文献和实验Preparation of HeLa Nuclear Extracts
Promoter-specific in vitro transcription by RNA polymerase II is now achievable with cell extracts prepared from a variety of animal and plant sources (1 –3 ). For extracts prepared from mammalian cells, HeLa cells provide many advantages
Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing
an obstacle until in vitro transcription with bacteriophage RNA polymerases (3 ) was adopted for this purpose. Another useful development was a nuclear extract preparation procedure that was initially developed for in vitro transcription studies
Largescale nuclear extract preparation
,Brenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Biol.Vol.10,P.777-781 I.Homogenize cells or tissue 1.Wash commercially available HeLa cells (30g)or minced in 3mm pieces
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