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- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Collagen Type V Alpha 2 (COL5a2)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPC152Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 54.1kDa
Predicted isoelectric point 7.3
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Ⅴ型胶原α2(COL5α2)重组蛋白
Subcellular Location Secreted, extracellular space, extracellular matrix.
Residues Cys82~Glu308 (Accession # P51861) with two N-terminal Tags, His-tag and GST-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-CADPVTPPG ECCPVCSQTP GGGNTNFGRG RKGQKGEPGL VPVVTGIRGR PGPAGPPGSQ GPRGERGPKG RPGPRGPQGI DGEPGVPGQP GAPGPPGHPS HPGPDGLSRP FSAQMAGLDE KSGLGSQVGL MPGSVGPVGP RGPQGLQGQQ GGAGPTGPPG EPGDPGPMGP IGSRGPEGPP GKPGEDGEPG RNGNPGEVGF AGSPGARGFP GAPGLPGLKG HRGHKGLE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ⅴ型胶原α2(COL5α2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验人整合素αVβ3(ITG αⅤβ3)酶联免疫分析(ELISA)
人整合素αVβ3(ITG αⅤβ3) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 整合素αVβ3(ITG αⅤβ3) 的 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 人 整合素αV β 3 ( ITG αⅤβ 3 ) 水平。用纯化的抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 整合素αV β 3 ( ITG αⅤ
、Ⅳ型也作用蔗糖,Ⅴ型还作用异麦芽糖,也就是二个葡萄糖以α- 1, 6键结合的糖和泊雷糖,即作用葡萄糖和果糖以α- 1, 6键结合的糖,这点上与Ⅰ、Ⅱ型不同。这些肠粘膜的酶,在肠道内可能参于二糖的消化吸收。Ⅲ、Ⅳ型以其可将蔗糖分解成葡萄糖和果糖这点是与蔗糖酶以至果糖苷酶具有同样的反应。
,或者可以表达以产生重组蛋白。pET-32α(+)载体系列用于表达与109 aa Trx•Tag™硫氧还蛋白融合的肽序列。它属于一种表达系统,可在其激活后产生所需的蛋白质。插入原核宿主细胞。原核细胞如大肠杆菌因为它们廉价,快速的生物量积累和简单的工艺规模扩大,因此是外源蛋白表达的首选宿主。在本文中,我们将重点总结这种在我们的实验室中使用的有用技术。表达后pET-32α(+)载体的构建,蛋白质表达和蛋白质纯化将在本文中详细描述。获得用于构建载体的外源DNA片段插入载体的DNA通常可以使用聚合酶链式反应(PCR
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