- 询价
- 沪震生物
- 进口/国产
- hz047Hu01
- 2025年07月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Ki-67 Protein (Ki67P)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPC047Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 18.3kDa
Predicted isoelectric point 6.7
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Ki-67蛋白(Ki67P)重组蛋白
Subcellular Location Nucleus. Chromosome.
Residues Met2815~Pro2928 (Accession # P46013) with two N-terminal Tags, His-tag and S-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- MTDDKT TKIPCKSSPE LEDTATSSKR RPRTRAQKVE VKEELLAVGK LTQTSGETTH TDKEPVGEGK GTKAFKQPAK RKLDAEDVIG SRRQPRAPKE KAQPLEDLAS FQELSQTP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ki-67蛋白(Ki67P)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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- 询问日期
文献和实验baibai17 今天开题，导师问了个问题：ki67为什么叫ki67？当场傻住······恳请各位老师指教！谢谢。 keavin521 The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67 (Gerdes et al., 1983),which was generated by immunizing mice
【求助】谢谢，请问ki67、PCNA、P53的英文全称是什么？
东德公民 谢谢，请问ki67、PCNA、P53的英文全称是什么？加急，谢谢 zhujoker p53 ：also known as protein 53 or tumor protein 53, is a transcription factor which in humans is encoded by the TP53 gene。 p53 is important in multicellular organisms
STM：广州医科大学刘铭/谢茂彬合作发现肝细胞癌的新潜在治疗靶点 Claudin 6
菲尼处理会上调 CLDN6 及相关谱系标志物的表达量，但下调增殖标志物 Ki67，表明上调 CLDN6 可能是肝癌细胞的一种应激策略，使自身进入低增殖速率的耐药状态。在分子水平上，CLDN6 可以上调 Hippo 通路转录因子 YAP1 和 TAZ 的表达量，并下调 YAP1 S127 和 S397 位点的抑制性磷酸化水平，从而活化 Hippo 通路。作者发现 CLDN6 可以与 YAP1 竞争性结合 TJP2，后者作为紧密连接复合体中的胞质支架蛋白，可以结合 YAP1 并诱导其降解。数据
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